Generated by analyze_mth938.py. Every number below is fetched at run time from
the InterPro, UniProt and QuickGO REST APIs; re-run to refresh.
The AAMDC review concludes that no molecular function is currently justifiable for
this protein. That is a negative claim. This analysis tests it the only way a
negative claim can be tested: enumerate the whole protein family and ask whether
any member has an experimentally supported molecular function that could transfer.
Fetched from the InterPro API at run time:
Members enumerated below are the reviewed (Swiss-Prot) entries carrying Pfam
PF04430 — a small, manually curated subset of all proteins with the signature,
chosen because only reviewed entries carry curated function statements and GO
annotations worth counting.
| Reviewed family members | 13 |
|---|---|
| GO annotations successfully retrieved for | 13 of 13 |
| With any experimental MF term (excluding bare protein binding) | 0 |
With an explicit GO:0003674 ND (curator: no function known) |
4 |
| With a UniProt CATALYTIC ACTIVITY block | 0 |
No member of this family carries any experimentally supported molecular
function term. The absence of an MF annotation on AAMDC is therefore not an
oversight specific to this gene: it is a property of the entire family.
The family is not entirely uncharacterised. 7 of its 13
reviewed members carry a UniProt FUNCTION statement about mitochondrial
complex I assembly, and all of them are NDUFAF3 entries. That branch is an
assembly factor for mitochondrial complex I, consistently across every
organism in which it has been studied; the Drosophila entry cites experimental
evidence (PubMed:34386730).
This is informative in a specific way. NDUFAF3 is an assembly factor, not an
enzyme: it has no catalytic activity, it binds subunits and helps build a complex,
and it is not part of the finished complex. Consistent with that, no member of this
family - including NDUFAF3 itself - carries a CATALYTIC ACTIVITY block or an
experimental molecular function term.
So the family signal does not point toward a hidden enzymatic activity waiting to
be found for AAMDC. It points toward a protein-assembly / chaperone-like role, which
is a class of function GO struggles to express as a molecular function in any case
(there is no assembly-chaperone MF term; see the AAGAB review in this campaign).
That makes the AAMDC review's refusal to assign an MF the right call, and it also
suggests where to look experimentally: what complex, if any, does AAMDC help
assemble?
| Accession | Entry | Organism | Len | Catalytic block | GO retrieved | Experimental MF |
|---|---|---|---|---|---|---|
| Q32PA8 | AAMDC_BOVIN | Bos taurus | 122 | no | yes | — |
| Q2HJI2 | NDUF3_BOVIN | Bos taurus | 184 | no | yes | — |
| Q502H1 | AAMDC_DANRE | Danio rerio | 122 | no | yes | — (MF=ND) |
| A1L1F1 | NDUF3_DANRE | Danio rerio | 174 | no | yes | — (MF=ND) |
| Q9W1H9 | NDUF3_DROME | Drosophila melanogaster | 196 | no | yes | — |
| P18152 | MP13_FRATH | Francisella tularensis subsp. holarctica (strain LVS) | 123 | no | yes | — |
| Q9BU61 | NDUF3_HUMAN | Homo sapiens | 184 | no | yes | — |
| Q9H7C9 | AAMDC_HUMAN | Homo sapiens | 122 | no | yes | — |
| Q8R0P4 | AAMDC_MOUSE | Mus musculus | 122 | no | yes | — (MF=ND) |
| Q9JKL4 | NDUF3_MOUSE | Mus musculus | 185 | no | yes | — (MF=ND) |
| O08776 | NDUF3_RAT | Rattus norvegicus | 185 | no | yes | — |
| P29962 | Y1784_RHOCB | Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) | 124 | no | yes | — |
| Q6DFN1 | NDUF3_XENTR | Xenopus tropicalis | 183 | no | yes | — |