Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Towards a proteome-scale map of the human protein-protein interaction network.
Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation.
An empirical framework for binary interactome mapping.
An atlas of combinatorial transcriptional regulation in mouse and man.
ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function.
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ASB9 binds and ubiquitinates mitochondrial creatine kinase.
"The overexpression of ASB9, but not ASB9DeltaSOCS, induces ubiquitination of uMtCK."
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ASB9 colocalizes with uMtCK in mitochondria.
"ASB9 and ASB9DeltaSOCS can interact and colocalise with uMtCK in the mitochondria."
Next-generation sequencing to generate interactome datasets.
Multimeric complexes among ankyrin-repeat and SOCS-box protein 9 (ASB9), ElonginBC, and Cullin 5: insights into the structure and assembly of ECS-type Cullin-RING E3 ubiquitin ligases.
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ASB9-EloBC binds Cul5 but not Cul2.
"We find that ASB9 is unstable alone but forms a stable ternary complex with EloBC that binds with high affinity to the Cullin 5 N-terminal domain (Cul5NTD) but not to Cul2NTD."
A proteome-scale map of the human interactome network.
How the ankyrin and SOCS box protein, ASB9, binds to creatine kinase.
Phospho-tyrosine dependent protein-protein interaction network.
Architecture of the human interactome defines protein communities and disease networks.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Structure and dynamics of the ASB9 CUL-RING E3 Ligase.
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Cryo-EM of CKB bound to ASB9-ELOB/C.
"Here we report cryo-EM data for the substrate, creatine kinase (CKB) bound to ASB9-ELOB/C, and for full-length CUL5 bound to the RING protein, RBX2, which binds various E2s."
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligases.
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Reconstituted ASB9-CUL5-RBX2 ubiquitylates CKB.
"Here we have assembled and purified the Ankyrin repeat and SOCS Box protein 9 CUL5 RBX2 ligase (ASB9-CRL) in vitro and show how it ubiquitylates one of its substrates, CKB."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Multimodal cell maps as a foundation for structural and functional genomics.
NEDD8:AcM-UBE2F binds CRL5 E3 ubiquitin ligase complex
AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex
CAND1 binds cytosolic CRL E3 ubiquitin ligases
COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
The Mechanism of Histone Ubiquitylation by the ASB9-CUL5 Ubiquitin Ligase.
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ASB9-CRL5 polyubiquitylates histones H3 and H4.
"Here, we characterized the ASB9-CRL5 ubiquitylation of histones and show that histones histone 3 (H3) and histone 4 (H4) are polyubiquitylated by the ASB9-CRL5 whereas histones Histone 2A and Histone 2B are much poorer substrates."
ASB9 promotes ubiquitin-mediated degradation of TNP2 to facilitate histone-to-protamine transition in humans and mice.
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A testis-specific ASB9-CRL5 complex degrades TNP2.
"Mechanistically, we found that ASB9 assembles a testis-specific Cullin-RING ligase (CRL) complex-TNP2-ASB9-ELOB/C-CUL5-RBX1-that mediates the ubiquitin-dependent degradation of TNP2 to facilitate the histone-to-protamine transition during spermiogenesis."
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ASB9 deficiency causes TNP2 retention in humans and mice.
"In this study, we investigated the role of the ankyrin repeat-containing SOCS box protein 9 (ASB9) during spermiogenesis and found that its deficiency causes TNP2 retention, leading to a failure of the histone-to-protamine transition in both humans and mice."
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ASB9 deficiency causes male infertility.
"This disruption consequently causes male infertility, characterized by sperm head malformation and impairments in fertilization and early embryonic development."
Ubiquitin protein E3 ligase ASB9 suppresses proliferation and promotes apoptosis in human spermatogonial stem cell line by inducing HIF1AN degradation.
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HIF1AN binds ASB9 and its re-expression reverses ASB9 overexpression effects in SSCs.
"Based on the results of protein co-immunoprecipitation, HIF1AN had direct interaction with ASB9, and re-expression of HIF1AN partially restored the cell proliferation and apoptotic changes caused by ASB9 deficiency."
UniProt entry Q96DX5 (ASB9_HUMAN)
Affinage mechanistic annotation for ASB9 (human)
Reactome R-HSA-8952039: NEDD8:AcM-UBE2F binds CRL5 E3 ubiquitin ligase complex
Reactome R-HSA-8952044: AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex
Reactome R-HSA-8955241: CAND1 binds cytosolic CRL E3 ubiquitin ligases
Reactome R-HSA-8955289: COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
Reactome R-HSA-8956040: COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes