Use of the ND evidence code for Gene Ontology (GO) terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Genome-wide identification of fungal GPI proteins.
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A genome-wide computational screen for a C-terminal GPI attachment consensus, an N-terminal secretion signal and absence of internal transmembrane domains identified only 33 GPI-protein candidates in the S. pombe genome; adg2/SPAC19G12.16c is among them, the basis for its cell-surface / GPI-anchored classification.
"only 33 GPI candidates were identified"
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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High-throughput YFP-tagging localization of ~4,431 S. pombe proteins; the per-protein dataset assigns adg2 to the endoplasmic reticulum, the experimental basis for the UniProt ER localization (ECO:0000269|PubMed:16823372).
"we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein"
The cell cycle-regulated genes of Schizosaccharomyces pombe.
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adg2 is a member of the nine-gene, Ace2-regulated "Eng1 cluster" of cell-cycle-regulated genes described as involved in cell separation, where adg1 and adg2 are annotated as cell surface glycoproteins alongside glucan hydrolases (eng1, agn1, adg3).
"The Eng1 cluster (Figure 8C) contains nine genes, and these are involved in cell separation. The genes are adg1 and adg2 (cell surface glycoproteins)"
Deciphering the transcriptional-regulatory network of flocculation in Schizosaccharomyces pombe.