trpE (PA0609, P20580) review notes

Literature summary

P. aeruginosa has two anthranilate synthases; trpE/trpG make the tryptophan enzyme PMID:2153661, and chromosomal trpE deletion gives PMID:2153661.
TrpEG is the tryptophan anthranilate synthase, PhnAB the pyocyanin one PMID:23449919; PMID:23449919.
Anthranilate synthase catalyses the first step shared by tryptophan and PQS synthesis [PMID:23449919 "PQS biosynthesis requires conversion of the\ncentral metabolite chorismate to anthranilate by anthranilate synthase"; "This reaction is also the first step in tryptophan biosynthesis"].
UniProt: reaction chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate (RHEA:21732, EC 4.1.3.27); heterotetramer of TrpE and TrpG; anthranilate synthase component I family.

Literature search (2026-11-05): PubMed MCP and NCBI E-utilities failed all session; Europe PMC search was
intermittent. Based on PMID:2153661 (abstract) and PMID:23449919 (full text, cached), the UniProt record, and
QuickGO for the seeds.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002599563	GO:0000162 L-tryptophan biosynthetic process	8	182 (95 taxa)	GO:0000162, IMP, PMID:2153661	SOUND	Chromosomal trpE knockout is a tryptophan auxotroph; trpE is explicitly the tryptophan (not pyocyanin) anthranilate synthase.	B. subtilis TrpE P00895 (IDA PMID:4886289, IMP PMID:5331787), S. cerevisiae TRP2 (SGD S000000892), S. pombe SPCC1442.09, Arabidopsis AT5G05730, M. tuberculosis P9WFX3, and plant/other UniProt Q94GF1, Q9XJ29	Yes; a deeply conserved node with multiple experimental seeds (e.g. B. subtilis TrpE IDA, QuickGO 2026-11-05)

No source_defects: the PseudoCAP row is sound and the IBD is sound for this broad, well-characterised enzyme
family. Only two trpE rows are PseudoCAP (both from PMID:2153661); the GO:0004049 IMP row is genetic rather than a
direct enzyme assay but the activity assignment is correct, so it is accepted (not flagged).

Action summary

5 rows, all ACCEPT (GO:0000162 IBA/IEA/IMP; GO:0004049 IEA/IMP).