Source method: ProtNLM2. Source version: live API snapshot 2026-09-09T03:00:51.831347+00:00. Accession: Q1K772. Raw source: NCU04302-protnlm-source.json.
Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins
| Exact claim span | Assessment | Rationale |
|---|---|---|
| Accepts the ubiquitin from the E1 complex | PLI (0) | The Ubc9 subfamily accepts activated SUMO. The literal ubiquitin substrate specifies the wrong E2 subfamily. |
| catalyzes its covalent attachment to other proteins | PLI (0) | “its” refers to ubiquitin in the emitted sentence. Ubc9 catalyzes SUMO attachment, so the stated substrate-specific reaction is incorrect. |
The conserved E2 reaction pattern is plausible only after changing the modifier to SUMO; that correction is not silently substituted for the original claim. The generic transferase GO prediction is separately LSP.
Modifier-specific sequence assignments are “DR CDD; cd23798; UBCc_UBE2I; 1.” and “DR FunFam; 3.10.110.10:FF:000035; SUMO-conjugating enzyme ubc9; 1.” Full-length comparisons give 75.0% identity to fission-yeast Ubc9 and 62.42% to human Ubc9, compared with 42.0% to the recorded ubiquitin-E2 donor; reproducible results preserve all alignments and controls. PMID:12597774 directly characterizes SUMO transfer in the fission-yeast reference. The current GOA PAINT assertion at PANTHER:PTN000629675 places the target in the SUMO E2 lineage; PROSITE identifies a UBC core at residues 3–156 and an active-site cysteine at 92. PMID:9435231(https://pubmed.ncbi.nlm.nih.gov/9435231/) establishes recombinant yeast and mammalian Ubc9 thioesters with SUMO. This is a conserved-family inference for the target, not an NCU04302 biochemical assay. The recorded phmmer donor is Arabidopsis UBC2 (P42745), and its current reviewed function text is identical to the emitted paragraph; that record attributes ubiquitin conjugation to PMID:16339806. This exact textual agreement does not establish the internal mechanism of prediction. The preserved donor record confirms that this is a different E2 specificity, not simply a synonym for SUMO.
Error type: PARALOG_OVERANNOTATION. Donor record: NCU04302-donor-P42745.json.
{
"commentType": "FUNCTION",
"texts": [
{
"evidences": [
{
"evidenceCode": "ECO:0008006",
"id": "ProtNLM2",
"properties": [
{
"key": "model_score",
"value": "0.19"
},
{
"key": "phmmer_accession",
"value": "P42745"
},
{
"key": "phmmer_score",
"value": "127.9"
}
],
"source": "Google"
}
],
"value": "Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins"
}
]
}