Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Human METTL20 is a mitochondrial lysine methyltransferase that targets the β subunit of electron transfer flavoprotein (ETFβ) and modulates its activity.
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ETF is a mobile electron carrier that shuttles electrons from matrix FAD-containing dehydrogenases to the membrane-bound ETF quinone oxidoreductase, and is the third major provider of electrons to the respiratory-chain ubiquinone pool after complexes I and II. METTL20/ETFBKMT trimethylates ETFB Lys-200/Lys-203 near the recognition loop, reducing electron uptake from MCAD and GCDH.
"it is the third major provider of electrons to the ubiquinone pool of the mitochondrial respiratory chain"
Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
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ETFB interacts with ETFA and ETFRF1/LYRM5; LYRM5 acts as an ETF deflavinase, removing FAD from the electron-transferring flavoprotein that shuttles electrons to coenzyme Q.
"LYRM5 interacts with and deflavinates"
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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Proteome-scale BioPlex AP-MS interactome; source of the IntAct-curated ETFB interactions with ETFA and ETFRF1.
"BioPlex 3.0, results from affinity purification"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
S1P defects cause a new entity of cataract, alopecia, oral mucosal disorder, and psoriasis-like syndrome.
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MBTPS1/S1P is a mitochondrial protein that forms a trimeric complex with ETFA/ETFB, enhancing ETF flavination (FAD incorporation) and stabilizing the heterodimer; loss impairs mitochondrial respiration and fatty-acid beta-oxidation.
"protein that forms a trimeric complex with ETFA/ETFB. S1P enhances ETFA/ETFB"
Multimodal cell maps as a foundation for structural and functional genomics.
Mutations and polymorphisms of the gene encoding the beta-subunit of the electron transfer flavoprotein in three patients with glutaric acidemia type II.
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Beta-ETF (ETFB) mutations cause glutaric acidemia type II; the R164Q mutation reduces electron transfer activity, and wild-type beta-ETF cDNA restores beta-oxidation flux in patient fibroblasts.
"cDNA complements the genetic defect and restores the beta-oxidation flux to"
cDNA cloning and mitochondrial import of the beta-subunit of the human electron-transfer flavoprotein.
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The cloned beta-ETF (255 aa) polypeptide contains the information necessary to reach the mitochondrial matrix via energy-dependent import, and lacks a cleavable leader peptide; the mRNA is abundant in liver, heart, and skeletal muscle.
"contains the information necessary to reach the mitochondrial matrix"
Assignment of Etfdh, Etfb, and Etfa to chromosomes 3, 7, and 13: the mouse homologs of genes responsible for glutaric acidemia type II in human.
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ETF (alpha/beta) is an obligatory electron acceptor for several dehydrogenases and is located in the mitochondrial matrix; electrons are transferred to the respiratory chain via ETFDH, and deficiency causes glutaric acidemia type II.
"obligatory electron acceptor for several dehydrogenases"
Three-dimensional structure of human electron transfer flavoprotein to 2.1-A resolution.
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The 2.1-A crystal structure of human ETF shows a heterodimer of three domains, the third contributed entirely by the beta subunit, with FAD in a cleft between subunits; ETF functions as an electron shuttle between primary flavoprotein dehydrogenases and ETF-ubiquinone oxidoreductase.
"third domain is made up entirely by the beta subunit"
Reducing equivalents from beta-oxidation of fatty acids transfer to ETF
ETFDH oxidises ETF (reduced) to ETF, reduces CoQ to CoQH2
ETFBKMT transfers 3xCH3 from 3xAdoMet to ETFB