grk7b notes

2026-05-09 review notes

Reviewed GOA, UniProt Q1XHL7, PMID:16787417, and PANTHER family cache. The direct core function is rhodopsin/light-activated opsin phosphorylation by a retinal GRK7 kinase, with broad kinase and signaling terms treated as less informative than the specific rhodopsin kinase activity.

Re-review 2026-09-29

• Validation initially failed: a stale row GO:0004703 (IEA, GO_REF:0000002) is no longer in GOA; removed it and reviewed the replacement row GO:0004703 (IEA, GO_REF:0000120, ARBA/InterPro) as MODIFY -> GO:0050254, consistent with the other broad kinase IEAs.
• Removed the previous NEW row for GO:0016056 G protein-coupled opsin signaling pathway. Human GRK7 carries GO:0016059 negative regulation of opsin-mediated signaling pathway (IDA/IMP, QuickGO), but the only in vivo test of this paralog is negative: PMID:33579376, and the retinal cone-recovery knockdown is grk7a PMID:16039565. The IBA GO:0009966 regulation of signal transduction is ACCEPTED at the family-node level instead.
• Rhodopsin kinase activity (GO:0050254; IDA, IBA, IEA, ISS) ACCEPT: grk7b is GRK7-2 of Wada et al. [PMID:16787417 "Screening of the ocular cDNAs identified two homologs for each of GRK1 (1A and 1B) and GRK7 (7-1 and 7-2)"; "The recombinant GRKs phosphorylated light-activated rhodopsin"]; UniProt sources both Rhea reactions from this paper (ECO:0000269). Abstract-only cache; deferred to the curator for the GRK7-2 assay details.
• Membrane rows now cite the SUBCELLULAR LOCATION line and the S-geranylgeranyl cysteine feature, plus the in situ localisation of GRK7-1 to cone outer segments (paralog-specific localisation of Grk7b is not shown; the anti-Grk7 antibody recognises both paralogs PMID:30372740). GO:0007165 signal transduction (IEA) KEEP_AS_NON_CORE -> MARK_AS_OVER_ANNOTATED.
• Cached PMID:33579376, PMID:16039565, PMID:34462505 and PMID:30372740; grk7b is the UV-cone-enriched paralog PMID:34462505. Added reference_review for all PMIDs, a standalone description, two questions and one experiment. Validation: 0 errors, 0 warnings.