Falcon (Edison Scientific) deep research report for CBF1
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CBF1/DREB1B is an AP2/ERF-family transcription factor that binds the CRT/DRE cis-element and activates the cold-induced COR/LTI/RD regulon; one of three tandemly arrayed CBFs (CBF1/CBF2/CBF3) at AT4G25490.
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CBF1 acts redundantly with CBF3 as a positive regulator of cold acclimation; antisense/RNAi reduction of CBF1+CBF3 lowers cold-induced freezing tolerance by ~60%.
Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
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CBF1 is one of >1500 Arabidopsis transcription factors classified by conserved DNA-binding domain (AP2/ERF), supporting DNA-binding transcription factor activity.
"Arabidopsis dedicates over 5% of its genome to code for more than 1500 transcription factors"
The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions.
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Cold-signaling phospholipase C/D pathways connect to the CBF pathway, the central genetic system for cold acclimation.
"The connection of these pathways with the CBF pathway, currently the most understood genetic system playing a role in cold acclimation, is discussed."
Physical and functional interactions of Arabidopsis ADA2 transcriptional coactivator proteins with the acetyltransferase GCN5 and with the cold-induced transcription factor CBF1.
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The DNA-binding domain of CBF1 binds directly to Arabidopsis ADA2 coactivator proteins, linking CBF1 to the SAGA-like ADA2/GCN5 complex.
"the DNA-binding domain of CBF1 (and of a related protein, TINY), rather than its transcriptional activation domain, can bind directly to the Arabidopsis ADA2 proteins."
Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon.
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CBF1 (with CBF3) is a positive regulator of cold acclimation; RNAi/antisense lines are defective in cold acclimation but not in constitutive freezing tolerance.
"CBF1 and CBF3 RNAi lines are defective in cold acclimation but are not affected in their constitutive freezing tolerance."
Plasma Membrane CRPK1-Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response.
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Phosphorylated 14-3-3 proteins move to the nucleus and interact with and destabilize CBF proteins to fine-tune cold signaling.
"they interact with and destabilize the key cold-responsive C-repeat-binding factor (CBF) proteins."
Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit.
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Recombinant CBF1 binds the C-repeat/DRE element in gel-shift assays.
"Binding of CBF1 to the C-repeat/DRE was demonstrated in gel shift assays using recombinant CBF1 protein expressed in Escherichia coli."
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CBF1 activates transcription of CRT/DRE-driven reporters but not mutant elements in yeast; acts as a transcriptional activator.
"expression of CBF1 in yeast was found to activate transcription of reporter genes containing the C-repeat/DRE as an upstream activator sequence but not mutant versions of the DNA element."
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CBF1 has an AP2 DNA-binding domain, a potential NLS, and an acidic activation domain.
"the protein has a molecular mass of 24 kDa, a potential nuclear localization sequence, and a possible acidic activation domain."
Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance.
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CBF1 overexpression induces COR gene expression and increases freezing tolerance of non-acclimated plants; CBF1 is a regulator of cold acclimation.
"Increased expression of Arabidopsis CBF1, a transcriptional activator that binds to the CRT/DRE sequence, induced COR gene expression and increased the freezing tolerance of nonacclimated Arabidopsis plants."
Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis.
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DREB1 (= CBF) factors act in the low-temperature pathway, separate from the DREB2 drought pathway; DREB proteins bind DRE and activate transcription.
"two independent families of DREB proteins, DREB1 and DREB2, function as trans-acting factors in two separate signal transduction pathways under low-temperature and dehydration conditions, respectively."
An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression.
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DREB1B (= CBF1) is part of a three-gene cold-induced DRE/CRT-binding family induced mainly by cold, not osmotic stress.
"the 3 DREB1 genes are induced mainly by cold stress but not by osmotic stress in leaves, roots, and stems."