Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Fe(III)-mediated cellular toxicity.
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The PmrA/PmrB system is required for Fe(III) resistance in Salmonella and E. coli
"Fe(III) exhibits microbicidal activity towards strains of Salmonella enterica, Escherichia coli and Klebsiella pneumoniae defective in the Fe(III)-responding PmrA/PmrB signal transduction system"
A Genome-wide view of the Escherichia coli BasS-BasR two-component system implicated in iron-responses.
Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes.
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The arn operon is induced by BasR (PmrA) in E. coli in response to Fe(3+)
"E. coli K-12 induces PmrA-activated gene transcription and polymyxin B resistance in response to Fe(3+), but that it is blind to the low Mg(2+) signal"
Global topology analysis of the Escherichia coli inner membrane proteome.
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ArnF (YfbJ) inner membrane localization confirmed by C-terminal GFP/PhoA fusion topology analysis
"Using C-terminal tagging with the alkaline phosphatase and green fluorescent protein, we established the periplasmic or cytoplasmic locations of the C termini for 601 inner membrane proteins"
An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli.
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ArnF (PmrM) deletion causes polymyxin sensitivity and loss of L-Ara4N modification of lipid A
"the pmrL and the pmrM deletion mutants (as well as the double deletion mutant) were sensitive to 15 μg/ml polymyxin"
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Over 95% of L-Ara4N-modified lipid A species are lost in the arnF mutant
"In the pmrL or pmrM deletion mutants, over 95% of each of the L-Ara4N-modified lipid A species was missing"
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ArnF is required for flipping undecaprenyl phosphate-alpha-L-Ara4N to the periplasmic face of the inner membrane
"PmrL and PmrM could specifically function to flip undecaprenyl phosphate-alpha-L-Ara4N from the cytosolic to the periplasmic side of the inner membrane, possibly functioning as a heterodimer"
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Sulfo-NHS-biotin labeling demonstrates 4-5 fold reduced periplasmic accessibility of substrate in arnF mutant
"At the 4-h time point, there was a 4-5-fold reduction in ratio of the monoisotopic peak areas for biotinylated undecaprenyl phosphate-alpha-L-Ara4N compared with unmodified undecaprenyl phosphate-alpha-L-Ara4N"
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ArnF is indispensable and cannot be complemented by ArnE alone
"polymyxin resistance was recovered in the pmrM deletion mutant AY101 by transforming with pWSK29-PmrM"
Deep research summary for arnF (Falcon/Edison Scientific Literature)
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ArnE/ArnF are placed as the flipping step between donor synthesis (ArnB/C/A/D) and transfer to lipid A (ArnT) in the L-Ara4N modification pathway
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ArnD is the deformylase that produces the ArnE/ArnF substrate (C55P-Ara4N) from C55P-Ara4FN, confirmed by Munoz-Escudero et al. 2023
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Polyphosphate kinase (PPK) regulates Arn pathway expression via BasRS during starvation (Baijal et al. 2024)