Gene Ontology annotation through association of InterPro records with GO terms

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/mobA/mobA-uniprot.txt

UniProtKB reviewed entry for mobA

Reviewed UniProt/HAMAP assigns Q88HA3 Mo-MPT guanylylation, EC 2.7.7.77, producing Mo-MGD.

"CC -!- FUNCTION: Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) CC cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine CC dinucleotide (Mo-MGD) cofactor. {ECO:0000255|HAMAP-Rule:MF_00316}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Mo-molybdopterin + GTP + H(+) = Mo-molybdopterin guanine CC dinucleotide + diphosphate; Xref=Rhea:RHEA:34243, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:71302, CC ChEBI:CHEBI:71310; EC=2.7.7.77; Evidence={ECO:0000255|HAMAP- CC Rule:MF_00316};"

file:PSEPK/mobA/mobA-goa.tsv

QuickGO GOA annotations for mobA

The fetched table contains the exact MobA activity annotation reviewed here.

"UniProtKB Q88HA3 mobA enables GO:0061603 molybdenum cofactor guanylyltransferase activity molecular_function ECO:0000501 IEA GO_REF:0000120 RHEA:34243|EC:2.7.7.77 160488 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) UniProt Molybdenum cofactor guanylyltransferase 20260706"

file:PSEPK/mobA/mobA-deep-research-openscientist.md

OpenScientist gene research for mobA

The report gives the conserved one-MGD guanylyltransferase reaction that is independently supported by reviewed target UniProt.

"GTP + Mo-MPT --(MobA, Mg2+)--> MGD + PPi"
The report contains concrete KT2440 locus errors for two upstream genes.

"whereas the core upstream Moco machinery is present: *moaA* (K03750, PP_2123), a *moeA*-family gene (PP_1294), and *mobA* itself (K03752, PP_3457, EC 2.7.7.77)."
The report presents an unsaved target-specific pairwise alignment as evidence.

"A global BLOSUM62 (Needleman–Wunsch) alignment of *P. putida* MobA (Q88HA3, 191 aa) against the experimentally characterized *E. coli* MobA (P32173, 194 aa) gives **29.6 % identity and 52.4 % similarity over 189 aligned positions** (alignment score 199)."
The report explicitly states that no direct Q88HA3 biochemical or structural measurements were found.

"**No *P. putida*-specific experimental data.** Q88HA3 is annotated PE = 3 (inferred from homology). Its catalytic activity, kinetics, substrate specificity, and structure have not been measured directly; all functional claims are transferred from orthologs."

file:interpro/panther/PTHR19136/PTHR19136-entries.csv

PANTHER representative entries for the molybdenum cofactor guanylyltransferase family

The local PANTHER export places KT2440 Q88HA3 in MobA subfamily PTHR19136:SF81.

"Q88HA3,Molybdenum cofactor guanylyltransferase,protein,160488,Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440),Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440),mobA,191,PTHR19136:SF81,MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE,True"
The same local subfamily export contains reviewed E. coli MobA P32173.

"P32173,Molybdenum cofactor guanylyltransferase,protein,83333,Escherichia coli (strain K12),Escherichia coli (strain K12),mobA,194,PTHR19136:SF81,MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE,True"