Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000002 supplied InterPro-derived annotations for cellulose synthase activity, cellulose biosynthetic process, and membrane localization based on the IPR005150 cellulose synthase domain.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000044 supplied the Golgi membrane localization annotation based on UniProt subcellular location vocabulary.

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000117 supplied ARBA-derived annotations for cytoplasm localization, response to cold, endomembrane system localization, and mannan synthase activity.

file:ARAHY/A0A444Z7V7/A0A444Z7V7-uniprot.txt

UniProtKB entry for A0A444Z7V7

UniProt classifies this protein in the glycosyltransferase 2 family, plant cellulose synthase-like D subfamily, with Golgi apparatus membrane localization and a cellulose synthase domain (PF03552) plus zinc finger RING domain (PF14570).

file:ARAHY/A0A444Z7V7/A0A444Z7V7-goa.tsv

QuickGO GOA annotations for A0A444Z7V7

GOA rows supplied the 8 existing annotations reviewed in this file.

file:ARAHY/A0A444Z7V7/A0A444Z7V7-deep-research-falcon.md

Deep research report on A0A444Z7V7 cellulose synthase domain-containing protein

Deep research confirmed no direct experimental studies exist for A0A444Z7V7 from peanut. Functional inference relies on conserved domain architecture and characterization of homologous CESA/CSL proteins in Arabidopsis, poplar, wheat, and other model plants.

file:ARAHY/A0A444Z7V7/A0A444Z7V7-bioinformatics/RESULTS.md

A0A444Z7V7 placement among CSLD and CESA proteins

In a MAFFT alignment and NJ tree, A0A444Z7V7 groups with Arabidopsis CSLD2 and CSLD3 (about 85% identity), inside the CSLD clade and apart from the CESAs. The catalytic aspartates, the nucleotide-sugar binding residues and the QxxRW motif are conserved.

PMID:32169960

Biochemical and Genetic Analysis Identify CSLD3 as a beta-1,4-Glucan Synthase That Functions during Plant Cell Wall Synthesis.

Reconstituted Arabidopsis CSLD3 is a UDP-glucose-dependent beta-1,4-glucan synthase that forms CESA6-like complexes.

PMID:21765420

A role for CSLD3 during cell-wall synthesis in apical plasma membranes of tip-growing root-hair cells.

CSLD3 localises to the apical plasma membrane of root hairs and supplies a (1->4)-beta-glucan synthase activity. A CSLD3 chimera with a CESA6 catalytic domain complements csld3.

PMID:21904114

Mannan synthase activity in the CSLD family.

Mutant and heterologous-expression evidence linking CSLD2, CSLD3 and CSLD5 to mannan synthesis.

PMID:21471331

The cooperative activities of CSLD2, CSLD3, and CSLD5 are required for normal Arabidopsis development.

csld2/3/5 mutant combinations have developmental defects and less mannan epitope.

PMID:11156607

KOJAK encodes a cellulose synthase-like protein required for root hair cell morphogenesis in Arabidopsis.

KOJAK/AtCSLD3 is required for root-hair elongation, and kjk root hairs rupture at the tip.

PMID:18768911

Functional analysis of the cellulose synthase-like genes CSLD1, CSLD2, and CSLD4 in tip-growing Arabidopsis cells.

CSLD2 and CSLD3 expression is elevated at reduced temperatures. CSLD2 is needed for root hairs, and CSLD1 and CSLD4 are needed for pollen-tube growth.