Gene Ontology annotation through association of InterPro records with GO terms

This reference supports IEA annotations based on InterPro domain assignments. For D7r5, the PBP/GOBP domain leads to odorant binding annotation, but this conflicts with experimental evidence showing D7r5 does not bind ligands.

Annotation inferences using phylogenetic trees

This reference supports IBA annotations. For D7r5, the phylogenetic inference from OBP family members incorrectly propagates sensory perception of smell, while the extracellular space annotation is appropriate.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

This reference supports the extracellular region annotation based on UniProt subcellular location. This is appropriate for D7r5.

PMID:16301315

Function and evolution of a mosquito salivary protein family

D7r5 is one of five short-form D7 proteins in Anopheles gambiae

"we expressed the five members of the small D7 family of the African malaria vector Anopheles gambiae"
Unlike D7r1-D7r4, D7r5 does NOT bind biogenic amines (serotonin, histamine, norepinephrine, adrenaline)

"four of these five short D7 proteins and the D7 long form bind serotonin with high affinity, as well as histamine and norepinephrine. The nonbinding D7 protein is poorly expressed in the salivary glands"
D7r5 is poorly expressed in salivary glands

"The nonbinding D7 protein is poorly expressed in the salivary glands"
D7r5 appears to be on the evolutionary path to becoming a pseudogene

"The nonbinding D7 protein is poorly expressed in the salivary glands and appears to be on the path to becoming a pseudogene"
The D7 family is distantly related to odorant-binding proteins but has evolved distinct functions in blood feeding

"The D7 salivary family of proteins is abundantly expressed in blood-feeding Diptera and is distantly related to the odorant-binding protein superfamily"