PIS1 (YPR113W, P06197) notes

Identity

• CDP-diacylglycerol--inositol 3-phosphatidyltransferase (PI synthase), EC 2.7.8.11; essential; six TM helices [UniProt:P06197].

Evidence

• Cloned by complementation PMID:6088233
• Purified and reconstituted PMID:3005284
• Kinetics of microsomal enzyme PMID:6274497
• Golgi activity PMID:8543066; MOM cytosolic face PMID:3005242

Curation decisions

• Core: GO:0003881 + PI biosynthesis, ER/Golgi/MOM membranes. All location annotations accepted except cytosol RCA (removed) and generic cytoplasm IEA (non-core).
• The MOM EXP from PMID:6274497 cannot be checked from the abstract (which describes microsomes); deferred to the curator.