Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
GOA annotations for Anopheles gambiae PGRPLC
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The fetched GOA line for the contested GO:0008745 IBA annotation propagates amidase activity through PANTHER:PTN002475783 and a mixed PGRP source set.
"UniProtKB A7UTA1 PGRPLC enables GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular_function ECO:0000318 IBA GO_REF:0000033 FB:FBgn0033327|FB:FBgn0037906|FB:FBgn0043578|PANTHER:PTN002475783|UniProtKB:Q96PD5|ZFIN:ZDB-GENE-050419-71|ZFIN:ZDB-GENE-071227-1|ZFIN:ZDB-GENE-071227-2 7165 Anopheles gambiae GO_Central AGAP005203-PC 20250731"
OpenScientist hypothesis investigation - PGRPLC amidase activity
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OpenScientist classified the GO:0008745 IBA amidase annotation as over-annotated and recommended removal.
"Verdict: Over-annotated."
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OpenScientist found that PGRPLC lacks two of the three zinc-binding residues required for PGRP amidase catalysis.
"PGRPLC lacks two of the three zinc-binding residues absolutely required for amidase catalysis"
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OpenScientist recommended removing the IBA GO:0008745 annotation from A7UTA1.
"The current annotation of GO:0008745 (N-acetylmuramoyl-L-alanine amidase activity) with IBA evidence should be **removed** from A7UTA1."
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Deep research summary for PGRPLC in Anopheles gambiae
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PGRPLC is the principal IMD pathway receptor in An. gambiae
"PGRPLC is the principal transmembrane receptor that activates the mosquito IMD/REL2 NF-kB pathway"
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RNAi knockdown demonstrated role in antibacterial defense
"Systemic antibacterial challenge survival (RNAi PGRPLC): Whole-gene KD decreased survival - E. coli, 50% mortality by day 4"
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PGRPLC is non-catalytic
"Despite the amidase fold, PGRP-LC functions as a non-catalytic pattern-recognition receptor in mosquitoes"
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Three isoforms show distinct ligand binding properties
"LC1 and LC3 bind insoluble polymeric DAP-PGN, while LC2 forms complexes with LC3 in the presence of TCT monomer"
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Located at midgut plasma membrane
"Functional analyses localize PGRPLC activity to the midgut epithelium with regionalized expression"