Research Report: Functional Annotation of *S. cerevisiae* **HSP82** (UniProt P02829) Falcon Edison Scientific Literature 25 citations 2 artifacts 2026-05-30T09:54:55.530562

The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.

You should prioritize authoritative reviews and primary scientific literature when conducting research. You can supplement
this with annotations you find in gene/protein databases, but these can be outdated or inaccurate.

We are specifically interested in the primary function of the gene - for enzymes, what reaction is catalyzed, and what is the substrate specificity? For transporters, what is the substrate? For structural proteins or adapters, what is the broader structural role? For signaling molecules, what is the role in the pathway.

We are interested in where in or outside the cell the gene product carries out its function.

We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.

Include evidence where possible. We are interested in both experimental evidence as well as inference from structure, evolution, or bioinformatic analysis. Precise studies should be prioritized over high-throughput, where available.

Research Report: Functional Annotation of S. cerevisiae HSP82 (UniProt P02829)

0) Target identity verification (mandatory)

Verified target: UniProt P02829 corresponds to the Saccharomyces cerevisiae cytosolic Hsp90 heat-inducible isoform Hsp82 (gene HSP82). A 2024 yeast-focused review explicitly maps Hsp82 to UniProt P02829 and distinguishes the paralog Hsc82 as a separate cytosolic Hsp90 isoform. (rios2024insightsintohsp90 pages 1-2)

Paralog distinction: Yeast has two closely related cytosolic Hsp90 isoforms, the constitutive Hsc82 and stress-inducible Hsp82, sharing ~97% amino-acid identity but exhibiting measurable functional differences. (girstmair2019thehsp90isoforms pages 1-2, rios2024insightsintohsp90 pages 1-2)

Family/domain alignment: Hsp82 is a canonical Hsp90-family chaperone with N-terminal ATP-binding domain, middle (client interaction) domain, and C-terminal dimerization domain including the conserved co-chaperone-binding motif at the C-terminus. (backe2023saccharomycescerevisiaeas pages 17-22, rios2024insightsintohsp90 pages 2-3, girstmair2019thehsp90isoforms media 835ffb5d)

Conclusion: The literature examined is consistent with the UniProt-provided identity: ATP-dependent molecular chaperone Hsp82 (HSP82; yeast Hsp90 heat-inducible isoform) in S. cerevisiae.

1) Key concepts and definitions (current understanding)

1.1 What Hsp82 does (primary function)

Hsp82 (Hsp90) is an ATP-dependent molecular chaperone that assists the late-stage folding, activation, and stability of a large set of specific “client” proteins (substrates), including many signaling regulators (notably protein kinases and transcription factors). (mercier2023hsp90mutantswith pages 1-2, nathan1999identificationofssf1 pages 1-2)

Hsp90/Hsp82 is best understood as a proteostasis hub rather than a general chaperone for all misfolded proteins: it supports selective refolding/activation (e.g., luciferase) while leaving other proteins relatively unaffected in comparable assays. (rios2024insightsintohsp90 pages 3-5)

1.2 ATPase-coupled conformational cycle (mechanistic definition)

Yeast Hsp90 (Hsp82/Hsc82) is a dimeric chaperone cycling between:
- an open state (C-terminally dimerized) and
- an ATP-induced closed state with additional N-terminal contacts.

Client loading, conformational closing, ATP hydrolysis/nucleotide exchange, and reopening are regulated by co-chaperones that bind distinct surfaces and bias the timing of transitions. (rios2024insightsintohsp90 pages 2-3, mercier2023hsp90mutantswith pages 1-2)

A central current concept is that dwell time in specific conformations can be more functionally determinative than absolute ATPase rate, emphasizing kinetic regulation of the cycle in vivo. (backe2023saccharomycescerevisiaeas pages 3-4, mercier2023hsp90mutantswith pages 1-2)

1.3 Co-chaperones (key definitions)

Yeast has ~14 Hsp90 co-chaperones (diverse domain architectures) that target clients, modulate ATPase activity, and stabilize or destabilize conformational states:
- Sti1/Hop cooperates with Hsp70 to target clients to open Hsp90 and must be released for progression. (rios2024insightsintohsp90 pages 2-3)
- Sba1/p23 stabilizes closed states and can influence product release steps. (rios2024insightsintohsp90 pages 2-3)
- Aha1 stimulates ATP hydrolysis via the middle domain. (rios2024insightsintohsp90 pages 2-3)
- Cdc37 preferentially targets protein kinases. (rios2024insightsintohsp90 pages 2-3)

Recent yeast genetics has refined distinct roles of Hch1 (destabilizes Hsp90–nucleotide interaction and inhibits early-cycle progression) and Cpr6 (stabilizes the closed conformation and counterbalances Hch1), providing a more granular step-wise view of cochaperone regulation. (mercier2023hsp90mutantswith pages 1-2, rios2024insightsintohsp90 pages 3-5)

1.4 “Chaperone code” (PTM-based regulation)

A modern framing is that Hsp90 activity is tuned by a combined “chaperone code” of co-chaperone binding and post-translational modifications (PTMs), which can alter cochaperone affinity, ATPase activity, and client fate. (rios2024insightsintohsp90 pages 3-5, backe2023saccharomycescerevisiaeas pages 17-22)

2) Recent developments (prioritizing 2023–2024)

2.1 2024: Yeast-centered synthesis of mechanism and in vivo function

A 2024 review focused on S. cerevisiae consolidates key mechanistic insights: closed-state adoption is rate-limiting, cochaperones partition folding steps, and isoform differences (Hsp82 vs Hsc82) largely map to the N-terminal domain, shaping ATPase activity and inhibitor sensitivity. (rios2024insightsintohsp90 pages 2-3, rios2024insightsintohsp90 pages 1-2)

The same review provides a quantitative summary of induction: Hsp82 is very low at 25–30°C and induced at 37°C to levels similar to Hsc82, consistent with a stress-specialized role. (rios2024insightsintohsp90 pages 1-2)

2.2 2023: Mutant dissection reveals cochaperones as “conformational pacemakers”

In 2023, Mercier et al. used yeast Hsp90 mutants defective in distinct cycle steps (“loading/closing/reopening”) to show that cochaperone genetic interactions align with closed-state formation and that drug sensitivity correlates with the conformational step affected (e.g., loading-defective mutants most sensitive to an Hsp90 inhibitor). (mercier2023hsp90mutantswith pages 1-2)

2.3 2024: Quantitative proteomics maps cycle-dependent client pools

A 2024 DIA-MS study profiled soluble proteomes of yeast expressing wild-type Hsp90 or cycle mutants, providing quantitative evidence for client pool partitioning by conformational states and offering a platform for “selective inhibition” concepts.

The study measured 2,482 proteins (~38% of the yeast proteome) and detected 350 proteins (14%) with significant abundance changes (log2 FC ≥ 1.5); ~73% of the strongest-changed proteins had prior connections to Hsp90 function. (rios2024quantitativeproteomicanalysis pages 1-2)

3) Functional annotation narrative (processes, pathways, localization)

3.1 Biological processes and pathway roles

Essential proteostasis for selective signaling proteins: Classic yeast genetics and biochemistry established that the HSP82/HSC82 family is essential (double disruption lethal), and that Hsp90 supports key signaling regulators. (kimura1994temperaturesensitivemutantsof pages 1-2, chang1994conservationofhsp90 pages 1-2)

MAPK/pheromone signaling: Hsp90 is required for basal and pheromone-induced MAPK signaling, with Ste11 (yeast Raf-equivalent MAPKKK) identified as a key endogenous Hsp90 client/substrate required for accumulation and pathway function. (louvion1998hsp90isrequired pages 1-2)

Steroid receptor maturation (heterologous client assays): Multiple classic studies used glucocorticoid receptor (GR) and other steroid receptors expressed in yeast as sensitive Hsp90 readouts, demonstrating that Hsp90 keeps receptors in an activatable state and supports hormone-dependent activation. (louvion1998hsp90isrequired pages 1-2, kimura1994temperaturesensitivemutantsof pages 1-2)

3.2 Cellular localization and where Hsp82 acts

Primary localization: Cytosolic Hsp90 (Hsp82/Hsc82) is broadly distributed during vegetative growth. (tapia2010hsp90nuclearaccumulation pages 1-2)

Condition-dependent nuclear accumulation: A key cell-biological finding is that Hsp90 (and the cochaperone Sba1/p23) accumulates in the nucleus in quiescent cells (glucose exhaustion) and in sporulating diploids; nuclear accumulation defects correlate with sporulation/spore-wall defects, and pharmacological inhibition (macbecin) similarly disrupts nuclear accumulation and spore development. (tapia2010hsp90nuclearaccumulation pages 1-2, tapia2010hsp90nuclearaccumulation pages 7-8)

4) Hsp82 vs Hsc82: specialization of two near-identical cytosolic isoforms

Multiple lines of evidence support that Hsp82 is tuned for stress conditions:
- Under nonstress conditions Hsc82 is ~10× more abundant; after heat shock Hsp82 is strongly induced so that levels become comparable. (girstmair2019thehsp90isoforms pages 1-2)
- Hsp82 is more thermally stable than Hsc82 (Tm ~60.4°C vs 57.1°C). (girstmair2019thehsp90isoforms pages 1-2, girstmair2019thehsp90isoforms media 00261b28)
- Hsc82 exhibits higher ATPase activity than Hsp82 at both 30°C and 37°C in comparative assays, and kinetic differences map largely to N-terminal-domain substitutions near the nucleotide pocket. (girstmair2019thehsp90isoforms pages 2-4, girstmair2019thehsp90isoforms pages 10-11, girstmair2019thehsp90isoforms media 7c8ac98c)

These findings support annotating Hsp82 as the heat-inducible, stress-resilient cytosolic Hsp90 isoform that preserves the core Hsp90 mechanism while shifting stability/dynamics. (girstmair2019thehsp90isoforms pages 1-2, rios2024insightsintohsp90 pages 1-2)

5) Applications and real-world implementations

5.1 Chemical-genetic screens and inhibitor studies in yeast

Yeast is widely used as a platform to probe Hsp90 mechanism and inhibitor response through drug sensitivity phenotypes and client reporters, and cross-species complementation (e.g., human Hsp90 isoforms expressed in yeast). (backe2023saccharomycescerevisiaeas pages 3-4, backe2023saccharomycescerevisiaeas pages 1-3)

A 2024 population-scale study explicitly used subtoxic Hsp90 inhibition as a screening proxy for environmental stress in growth/trait assays, demonstrating how Hsp90 perturbation can expose cryptic genetic variation and trait dependencies. (condic2024selectionforrobust pages 1-3, condic2024selectionforrobust pages 3-4)

5.2 Industrial fermentation and yeast domestication (2024 Science)

A 2024 Science study directly connects Hsp90 stress to economically important yeast traits: ethanol (a fermentation product) acts as a proteotoxic stressor that can compromise Hsp90-dependent regulators of sugar metabolism; domesticated beer and bread strains show increased robustness of maltose/maltotriose metabolism under Hsp90 stress, partly via gene duplications. (condic2024selectionforrobust pages 1-3, condic2024selectionforrobust pages 13-14)

In practical assay terms, the work demonstrates reproducible Hsp90 perturbation using radicicol at 4 μM and 10 μM, evaluated across 711 yeast strains and 12 metabolic traits. (condic2024selectionforrobust pages 1-3, condic2024selectionforrobust pages 3-4)

6) Quantitative summary table (recent statistics)

Finding/metric Value(s) Experimental context Source (first author, year, journal) URL Notes for annotation
Hsp82 vs Hsc82 sequence identity 97% amino-acid identity; 16 residue differences Comparative analysis of the two cytosolic yeast Hsp90 isoforms Girstmair, 2019, Nature Communications (girstmair2019thehsp90isoforms pages 1-2) https://doi.org/10.1038/s41467-019-11518-w Confirms HSP82 belongs to the Hsp90 family and is distinct from HSC82 despite very high similarity.
Hsp82 baseline abundance relative to Hsc82 under nonstress conditions Hsc82 ~10-fold higher than Hsp82 Nonstress expression in S. cerevisiae Girstmair, 2019, Nature Communications (girstmair2019thehsp90isoforms pages 1-2) https://doi.org/10.1038/s41467-019-11518-w Supports annotation of Hsp82 as the low-baseline, stress-inducible cytosolic Hsp90 isoform.
Hsp82 induction at elevated temperature At 37°C, Hsp82 rises to levels similar to Hsc82 Heat-induction of cytosolic Hsp90 isoforms Rios, 2024, Frontiers in Molecular Biosciences (rios2024insightsintohsp90 pages 1-2) https://doi.org/10.3389/fmolb.2024.1325590 Key evidence that HSP82 is the heat-inducible isoform.
Growth supported by reduced Hsp90 expression 1%–5% of wild-type expression still permits growth at optimal temperature, but not elevated temperature Genetic expression reduction of yeast cytosolic Hsp90 Rios, 2024, Frontiers in Molecular Biosciences (rios2024insightsintohsp90 pages 1-2) https://doi.org/10.3389/fmolb.2024.1325590 Indicates Hsp90 function is essential but quantitatively buffered under permissive conditions.
Essentiality of HSP82/HSC82 family Single deletion viable; double disruption lethal Classic yeast genetics of paralog pair Kimura, 1994, Molecular and General Genetics (kimura1994temperaturesensitivemutantsof pages 1-2) https://doi.org/10.1007/bf00285275 Important for functional annotation: at least one cytosolic Hsp90 isoform is required for viability.
Hsp82 melting temperature (Tm) 60.4 ± 0.5 °C Thermal stability comparison without nucleotide Girstmair, 2019, Nature Communications (girstmair2019thehsp90isoforms pages 1-2) https://doi.org/10.1038/s41467-019-11518-w Reflects enhanced thermotolerance of Hsp82, consistent with stress specialization.
Hsc82 melting temperature (Tm) 57.1 ± 0.2 °C Thermal stability comparison without nucleotide Girstmair, 2019, Nature Communications (girstmair2019thehsp90isoforms pages 1-2) https://doi.org/10.1038/s41467-019-11518-w Lower Tm than Hsp82 helps explain isoform specialization.
ATPγS effect on thermal stability ~+3 °C for both isoforms ATP analog binding during thermal unfolding analysis Girstmair, 2019, Nature Communications (girstmair2019thehsp90isoforms pages 1-2) https://doi.org/10.1038/s41467-019-11518-w Supports ATP-dependent stabilization of the Hsp90 fold.
Hsc82 ATPase activity relative to Hsp82 ~1.3-fold higher at 30°C; ~1.6-fold higher at 37°C Isoform enzymology comparison Girstmair, 2019, Nature Communications (girstmair2019thehsp90isoforms pages 2-4) https://doi.org/10.1038/s41467-019-11518-w Indicates isoform-specific ATPase tuning rather than different core family assignment.
Hsp82 refolding efficiency vs Hsc82 ~29% vs ~14% full refolding events Single-molecule mechanical assays Girstmair, 2019, Nature Communications (girstmair2019thehsp90isoforms pages 2-4) https://doi.org/10.1038/s41467-019-11518-w Quantifies superior resilience/refolding behavior of stress-induced Hsp82.
Proteome fraction broadly affected by Hsp90 inhibition ~10%–15% of proteins Review of yeast Hsp90 functional impact Rios, 2024, Genetics (rios2024quantitativeproteomicanalysis pages 1-2) https://doi.org/10.1093/genetics/iyae057 Shows Hsp90 is selective but still proteome-broad in influence.
Proteomics dataset size 2,482 proteins measured (~38% of yeast proteome) DIA-MS analysis of strains expressing wild-type or mutant Hsp90 Rios, 2024, Genetics (rios2024quantitativeproteomicanalysis pages 1-2) https://doi.org/10.1093/genetics/iyae057 Useful benchmark for the scale of experimentally observed Hsp90-dependent proteome effects.
Proteins significantly altered in Hsp90 mutant dataset 350 proteins (14% of measured set), using log2 fold change ≥ 1.5 Quantitative proteomics of 9 Hsp90 mutants vs wild type Rios, 2024, Genetics (rios2024quantitativeproteomicanalysis pages 1-2) https://doi.org/10.1093/genetics/iyae057 Supports annotation of Hsp90/Hsp82 as a major proteostasis hub affecting many clients/indirect targets.
Previously known Hsp90-linked proteins within altered set 257/350 (~73%) Cross-comparison of proteomic hits with prior Hsp90 literature Rios, 2024, Genetics (rios2024quantitativeproteomicanalysis pages 1-2) https://doi.org/10.1093/genetics/iyae057 Suggests many abundance changes reflect bona fide Hsp90-connected biology.
Number of Hsp90 mutants analyzed in proteomics study 9 mutants Mutants disrupting distinct steps of the Hsp90 cycle Rios, 2024, Genetics (rios2024quantitativeproteomicanalysis pages 1-2) https://doi.org/10.1093/genetics/iyae057 Supports state-specific/client-specific functional annotation.
Principal component clustering of mutant effects 3 primary clusters PCA of proteomic profiles from Hsp90-cycle mutants Rios, 2024, Genetics (rios2024quantitativeproteomicanalysis pages 1-2) https://doi.org/10.1093/genetics/iyae057 Suggests distinct conformational states support distinct client pools.
Industrial/domestication strain panel size 711 strains Comparative analysis of domesticated and wild yeast robustness under Hsp90 stress Condic, 2024, Science (condic2024selectionforrobust pages 1-3) https://doi.org/10.1126/science.adi3048 Strong recent evidence linking Hsp90 to real-world fermentation adaptation.
Number of metabolic traits screened 12 traits Hsp90 inhibition used as proxy for environmental/proteotoxic stress Condic, 2024, Science (condic2024selectionforrobust pages 1-3) https://doi.org/10.1126/science.adi3048 Demonstrates broad metabolic phenotyping framework for Hsp90-dependent robustness.
Radicicol concentrations used for Hsp90 perturbation 4 µM and 10 µM Low and moderate Hsp90 disruption in chemical-genetic screens Condic, 2024, Science (condic2024selectionforrobust pages 3-4) https://doi.org/10.1126/science.adi3048 Practical concentrations for yeast Hsp90-stress screening assays.
Genetic variance in maltose robustness explained by MAL gene duplications ≥60% of genetic variance Domestication/fermentation robustness under Hsp90 stress Condic, 2024, Science (condic2024selectionforrobust pages 13-14) https://doi.org/10.1126/science.adi3048 Indicates Hsp90-buffered variation has major quantitative consequences for industrial traits.
Diversity sampled in domestication analysis 73 genetic backgrounds; 10 ecological niches Population-scale metabolic robustness analysis Condic, 2024, Science (condic2024selectionforrobust pages 3-4) https://doi.org/10.1126/science.adi3048 Shows the Hsp90 effect was evaluated across broad yeast diversity.
Statistical significance for trait variability across isolates F test, P = 2.0585 × 10^-8 Variation in Hsp90-stress sensitivity across strains/traits Condic, 2024, Science (condic2024selectionforrobust pages 3-4) https://doi.org/10.1126/science.adi3048 Supports that Hsp90-dependent metabolic robustness differences are highly significant.
Hsp90 abundance can be reduced without viability at 25°C ~10-fold reduction tolerated at 25°C Classic Hsp90 abundance/viability genetics Nathan, 1999, PNAS (nathan1999identificationofssf1 pages 1-2) https://doi.org/10.1073/pnas.96.4.1409 Reinforces the idea that abundance reserve exists under permissive conditions, despite essentiality of the family.

Table: This table compiles key numeric findings relevant to functional annotation of yeast HSP82/Hsp90, including isoform identity, heat induction, essentiality thresholds, thermal and ATPase differences, proteome-wide effects, and recent industrial screening data.

7) Visual evidence (domain architecture and isoform comparison)

The following images from Girstmair et al. (2019) provide visual support for the canonical Hsp90 domain architecture and quantitative isoform comparison tables (thermal stability, ATPase/inhibitor binding): (girstmair2019thehsp90isoforms media 835ffb5d, girstmair2019thehsp90isoforms media 00261b28, girstmair2019thehsp90isoforms media 7c8ac98c)

8) Practical functional-annotation statements (evidence-backed)

Gene product: ATP-dependent molecular chaperone (Hsp90 family), cytosolic, heat-inducible isoform. (rios2024insightsintohsp90 pages 1-2, girstmair2019thehsp90isoforms media 835ffb5d)

Molecular function: ATP binding and ATPase-coupled conformational cycling that supports folding/activation/stabilization of select client proteins (notably kinases and transcription factors), coordinated by cochaperones. (rios2024insightsintohsp90 pages 2-3, mercier2023hsp90mutantswith pages 1-2)

Biological process: Proteostasis and regulation of signaling networks; essentiality at organismal level arises from dependence of key clients/pathways (e.g., MAPK pheromone pathway via Ste11). (louvion1998hsp90isrequired pages 1-2, kimura1994temperaturesensitivemutantsof pages 1-2)

Cellular component: Cytosol under vegetative growth; condition-dependent nuclear accumulation during quiescence/sporulation. (tapia2010hsp90nuclearaccumulation pages 1-2)

9) Notes on evidence limitations

Some requested details (e.g., yeast-specific inhibitor IC50 values, or a direct paper snippet explicitly stating the systematic locus name YPL240C) were not present in the extracted full-text evidence corpus used here; however, UniProt mapping to P02829 and the yeast isoform context are explicitly supported by recent review literature, and functional/locus context is supported by multiple classic and modern yeast studies. (rios2024insightsintohsp90 pages 1-2, kimura1994temperaturesensitivemutantsof pages 1-2)

References

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  2. (girstmair2019thehsp90isoforms pages 1-2): Hannah Girstmair, Franziska Tippel, Abraham Lopez, Katarzyna Tych, Frank Stein, Per Haberkant, Philipp Werner Norbert Schmid, Dominic Helm, Matthias Rief, Michael Sattler, and Johannes Buchner. The hsp90 isoforms from s. cerevisiae differ in structure, function and client range. Nature Communications, Aug 2019. URL: https://doi.org/10.1038/s41467-019-11518-w, doi:10.1038/s41467-019-11518-w. This article has 84 citations and is from a highest quality peer-reviewed journal.

  3. (backe2023saccharomycescerevisiaeas pages 17-22): Sarah J. Backe, Mehdi Mollapour, and Mark R. Woodford. saccharomyces cerevisiae as a tool for deciphering hsp90 molecular chaperone function. Essays in Biochemistry, 67:781-795, Sep 2023. URL: https://doi.org/10.1042/ebc20220224, doi:10.1042/ebc20220224. This article has 5 citations and is from a peer-reviewed journal.

  4. (rios2024insightsintohsp90 pages 2-3): Erick I. Rios, Isabel L. Hunsberger, and Jill L. Johnson. Insights into hsp90 mechanism and in vivo functions learned from studies in the yeast, saccharomyces cerevisiae. Frontiers in Molecular Biosciences, Feb 2024. URL: https://doi.org/10.3389/fmolb.2024.1325590, doi:10.3389/fmolb.2024.1325590. This article has 8 citations.

  5. (girstmair2019thehsp90isoforms media 835ffb5d): Hannah Girstmair, Franziska Tippel, Abraham Lopez, Katarzyna Tych, Frank Stein, Per Haberkant, Philipp Werner Norbert Schmid, Dominic Helm, Matthias Rief, Michael Sattler, and Johannes Buchner. The hsp90 isoforms from s. cerevisiae differ in structure, function and client range. Nature Communications, Aug 2019. URL: https://doi.org/10.1038/s41467-019-11518-w, doi:10.1038/s41467-019-11518-w. This article has 84 citations and is from a highest quality peer-reviewed journal.

  6. (mercier2023hsp90mutantswith pages 1-2): Rebecca Mercier, Danielle Yama, Paul LaPointe, and Jill L. Johnson. Hsp90 mutants with distinct defects provide novel insights into cochaperone regulation of the folding cycle. PLOS Genetics, 19:e1010772, May 2023. URL: https://doi.org/10.1371/journal.pgen.1010772, doi:10.1371/journal.pgen.1010772. This article has 14 citations and is from a domain leading peer-reviewed journal.

  7. (nathan1999identificationofssf1 pages 1-2): Debra F. Nathan, Melissa Harju Vos, and Susan Lindquist. Identification of ssf1, cns1, and hch1 as multicopy suppressors of a saccharomyces cerevisiae hsp90 loss-of-function mutation. Proceedings of the National Academy of Sciences of the United States of America, 96 4:1409-14, Feb 1999. URL: https://doi.org/10.1073/pnas.96.4.1409, doi:10.1073/pnas.96.4.1409. This article has 133 citations and is from a highest quality peer-reviewed journal.

  8. (rios2024insightsintohsp90 pages 3-5): Erick I. Rios, Isabel L. Hunsberger, and Jill L. Johnson. Insights into hsp90 mechanism and in vivo functions learned from studies in the yeast, saccharomyces cerevisiae. Frontiers in Molecular Biosciences, Feb 2024. URL: https://doi.org/10.3389/fmolb.2024.1325590, doi:10.3389/fmolb.2024.1325590. This article has 8 citations.

  9. (backe2023saccharomycescerevisiaeas pages 3-4): Sarah J. Backe, Mehdi Mollapour, and Mark R. Woodford. saccharomyces cerevisiae as a tool for deciphering hsp90 molecular chaperone function. Essays in Biochemistry, 67:781-795, Sep 2023. URL: https://doi.org/10.1042/ebc20220224, doi:10.1042/ebc20220224. This article has 5 citations and is from a peer-reviewed journal.

  10. (rios2024quantitativeproteomicanalysis pages 1-2): Erick I Rios, Davi Gonçalves, Kevin A Morano, and Jill L Johnson. Quantitative proteomic analysis reveals unique hsp90 cycle-dependent client interactions. Genetics, Apr 2024. URL: https://doi.org/10.1093/genetics/iyae057, doi:10.1093/genetics/iyae057. This article has 5 citations and is from a domain leading peer-reviewed journal.

  11. (kimura1994temperaturesensitivemutantsof pages 1-2): Yoko Kimura, Seiji Matsumoto, and Ichiro Yahara. Temperature-sensitive mutants of hsp82 of the budding yeast saccharomyces cerevisiae. Molecular and General Genetics MGG, 242:517-527, Mar 1994. URL: https://doi.org/10.1007/bf00285275, doi:10.1007/bf00285275. This article has 71 citations.

  12. (chang1994conservationofhsp90 pages 1-2): H.C. Chang and S. Lindquist. Conservation of hsp90 macromolecular complexes in saccharomyces cerevisiae. Journal of Biological Chemistry, 269:24983-24988, Oct 1994. URL: https://doi.org/10.1016/s0021-9258(17)31486-2, doi:10.1016/s0021-9258(17)31486-2. This article has 219 citations and is from a domain leading peer-reviewed journal.

  13. (louvion1998hsp90isrequired pages 1-2): Jean-François Louvion, Toufik Abbas-Terki, and Didier Picard. Hsp90 is required for pheromone signaling in yeast. Molecular biology of the cell, 9 11:3071-83, Nov 1998. URL: https://doi.org/10.1091/mbc.9.11.3071, doi:10.1091/mbc.9.11.3071. This article has 141 citations and is from a domain leading peer-reviewed journal.

  14. (tapia2010hsp90nuclearaccumulation pages 1-2): Hugo Tapia and Kevin A. Morano. Hsp90 nuclear accumulation in quiescence is linked to chaperone function and spore development in yeast. Molecular Biology of the Cell, 21:63-72, Jan 2010. URL: https://doi.org/10.1091/mbc.e09-05-0376, doi:10.1091/mbc.e09-05-0376. This article has 54 citations and is from a domain leading peer-reviewed journal.

  15. (tapia2010hsp90nuclearaccumulation pages 7-8): Hugo Tapia and Kevin A. Morano. Hsp90 nuclear accumulation in quiescence is linked to chaperone function and spore development in yeast. Molecular Biology of the Cell, 21:63-72, Jan 2010. URL: https://doi.org/10.1091/mbc.e09-05-0376, doi:10.1091/mbc.e09-05-0376. This article has 54 citations and is from a domain leading peer-reviewed journal.

  16. (girstmair2019thehsp90isoforms media 00261b28): Hannah Girstmair, Franziska Tippel, Abraham Lopez, Katarzyna Tych, Frank Stein, Per Haberkant, Philipp Werner Norbert Schmid, Dominic Helm, Matthias Rief, Michael Sattler, and Johannes Buchner. The hsp90 isoforms from s. cerevisiae differ in structure, function and client range. Nature Communications, Aug 2019. URL: https://doi.org/10.1038/s41467-019-11518-w, doi:10.1038/s41467-019-11518-w. This article has 84 citations and is from a highest quality peer-reviewed journal.

  17. (girstmair2019thehsp90isoforms pages 2-4): Hannah Girstmair, Franziska Tippel, Abraham Lopez, Katarzyna Tych, Frank Stein, Per Haberkant, Philipp Werner Norbert Schmid, Dominic Helm, Matthias Rief, Michael Sattler, and Johannes Buchner. The hsp90 isoforms from s. cerevisiae differ in structure, function and client range. Nature Communications, Aug 2019. URL: https://doi.org/10.1038/s41467-019-11518-w, doi:10.1038/s41467-019-11518-w. This article has 84 citations and is from a highest quality peer-reviewed journal.

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  19. (girstmair2019thehsp90isoforms media 7c8ac98c): Hannah Girstmair, Franziska Tippel, Abraham Lopez, Katarzyna Tych, Frank Stein, Per Haberkant, Philipp Werner Norbert Schmid, Dominic Helm, Matthias Rief, Michael Sattler, and Johannes Buchner. The hsp90 isoforms from s. cerevisiae differ in structure, function and client range. Nature Communications, Aug 2019. URL: https://doi.org/10.1038/s41467-019-11518-w, doi:10.1038/s41467-019-11518-w. This article has 84 citations and is from a highest quality peer-reviewed journal.

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Artifacts

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