Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
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gbcA and gbcB mutants grow on DMG but not on GB.
"strains with mutations in two genes, gbcA (PA5410) and gbcB (PA5411), were capable of growth on dimethylglycine (DMG), a catabolic product of GB, but not on GB itself"
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13C NMR shows gbcAB are needed for GB to DMG conversion.
"Subsequent nuclear magnetic resonance (NMR) experiments with 1,2-(13)C-labeled choline indicated that these genes are necessary for conversion of GB to DMG."
UniProt entry Q9HTF3
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GbcB is the ferredoxin reductase component of the GB monooxygenase.
"is the ferredoxin reductase component of the system"
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GbcB has a C-terminal 2Fe-2S ferredoxin-type domain with four cysteine ligands.
"/ligand="[2Fe-2S] cluster""
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods