PA3761 (Q9HXN4) review notes

NagE-family PTS enzyme IIBC (TCDB 4.A.1.1.15; NCBIfam TIGR01998 PTS-II-BC-nag;
PANTHER PTHR30009:SF4). EIIC (3..371, 8 TM helices), EIIB (383..466, active-site
Cys405, checked in the sequence), second EIIB-like domain (491..570) that UniProt
cautions lacks conserved residues. No EIIA domain; PA3760 is the fused
EI/HPr/EIIA partner.

Literature (no deep-research provider; own reading, 2026-11-05)

Johnson et al. 2008 (full text, PMC2542419): Biolog screen of 78 transporter
mutants. PMID:18833300 and PMID:18833300 The genes sit at the end of a
glucosamine utilisation operon. The mutant is listed under strain PAK.
E. coli NagE (P09323), the other node seed: GO:0015764 IDA PMID:4919472,
GO:0090586 IDA/EXP PMID:3056518, GO:0009401 IDA (EcoCyc; QuickGO 2026-11-05).
PubMed MCP returned API errors on 2026-11-05.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN000768523	GO:0015764 N-acetylglucosamine transport	2	14 (11 taxa)	GO:0015764, IMP, PMID:18833300 (PseudoCAP)	SOUND	Full text read: GlcNAc-specific growth defect plus GlcNAc induction, in a NagE-family EIIBC. A growth phenotype alone could be catabolic, but the family placement makes transport the right reading.	E. coli NagE P09323 (IDA PMID:4919472)	Yes; NagE IDA seed, and the node is a NagE subfamily clade

No PSEUDOCAP or GO_CENTRAL defect.

Other decisions

GO:0090563 (IBA, PTN002011274) and GO:0008982 (IEA) MODIFY to the GlcNAc
phosphocysteine PTS term GO:0090586, the term E. coli NagE carries by IDA.
GO:0019866 organelle inner membrane (InterPro2GO) is an organelle term
applied to a bacterium; MODIFY to plasma membrane.