GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:25416956

A proteome-scale map of the human interactome network.

High-throughput Y2H interactome mapping that detected SYCE3 interactions with HAUS1 and BLOC1S6. These interactions lack validation in the context of SYCE3's meiotic function.

PMID:27142324

Regulating the construction and demolition of the synaptonemal complex.

Review article providing general overview of SC assembly and regulation. Does not contain SYCE3-specific experimental data. Used as reference for ComplexPortal annotations.

PMID:31023827

A molecular model for self-assembly of the synaptonemal complex protein SYCE3.

Determined crystal structure of SYCE3 showing dimeric four-helical bundle (PDB 6H86)

"SYCE3 adopts a dimeric four-helical bundle structure that acts as the building block for concentration-dependent self-assembly into a series of discrete higher-order oligomers"
Identified SYCE3 self-assembly mechanism through staggered lateral and end-on interactions

"This is achieved through staggered lateral interactions between self-assembly surfaces of SYCE3 dimers and through end-on interactions that likely occur through intermolecular domain swapping between dimer folds"
Key residues W41 and Y44 mediate lateral self-assembly; P53 affects oligomerization

"mutation of Trp-41 and Tyr-44 residues to glutamate completely inhibited SYCE3 self-assembly, as determined by SEC-MALS, leaving a nonassembled 21-kDa dimer"
SYCE3 forms dimers, tetramers, and higher-order oligomers (up to dodecamers)

"SYCE3 assembly, particularly favoring formation of dodecamers of three laterally associated end-on tetramers"

PMID:31515488

Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.

High-throughput study of missense SNV effects on protein interactions. SYCE3-HAUS1 interaction tested.

PMID:32296183

A reference map of the human binary protein interactome.

HuRI reference interactome detected SYCE3 interactions with PKN1, KRT79, SGF29. These lack biological validation for SYCE3's meiotic function.

PMID:32814053

Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.

Neurodegenerative disease interactome study detected SYCE3 interactions with HTT, KLF11, NUP58, WFS1, PRPS1. These are unlikely to be physiologically relevant for SYCE3's meiotic function.

PMID:36635604

Structural maturation of SYCP1-mediated meiotic chromosome synapsis by SYCE3.

SYCE3 actively remodels SYCP1 tetramer lattice during early synapsis, not just stabilizes it

"SYCE3 has an essential role in actively remodelling SYCP1 tetramer lattices during the early stages of synapsis"
SYCE3 binds SYCP1 tetrameric core (alphaNcore) with approximately 170 nM affinity

"indicated a binding affinity of 170 ± 30 nM"
SYCE3 binding converts SYCP1 tetramers to 2:1 SYCP1-SYCE3 heterotrimers

"SYCP1 tetramers undergo conformational change into 2:1 heterotrimers on SYCE3 binding, removing their assembly interfaces and disrupting the SYCP1 lattice"
SYCE3 self-assembly provides tetramer-like interfaces for integrated SYCP1-SYCE3 lattice

"SYCE3 then establishes a new lattice by its self-assembly mimicking the role of the disrupted interface in tethering together SYCP1 dimers"
SYCE3 W41E/Y44E mutation blocks lateral self-assembly and integrated lattice formation

"The SYCE3 WY mutation separates the disruptive and integrative functions of SYCE3, triggering SYCP1 tetramer lattice disruption, whilst failing to form an integrated SYCP1-SYCE3 lattice"
Syce3WY/WY mice are infertile with severely disrupted SYCP1 assemblies

"Syce3WY/WY and Syce3Δ/Δ homozygotes exhibited severely reduced testis weights and epididymal sperm counts, with testis histology indicating a severe block in meiosis"
Syce3 null mice retain SYCP1 tetramer lattices but cannot form mature SC

"Syce3Δ/Δ captures the formation of SYCP1 tetramer lattices between axes, which cannot be remodelled in absence of SYCE3, so fail to develop into a mature SC"
SYCE3 recruits SYCE1-SIX6OS1 and SYCE2-TEX12 complexes (micromolar affinity)

"SYCE3 remodels and integrates into the SYCP1 lattice, establishing binding sites that cooperativity recruit SYCE1-SIX6OS1 and SYCE2-TEX12"
SYCE3 promotes fibrous assembly of SYCE2-TEX12

"SYCE3 promoted fibrous assembly of SYCE2-TEX12"