Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Cloning and expression of a plasma membrane cystine/glutamate exchange transporter composed of two distinct proteins.
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Original cloning identified xCT as the light chain forming heterodimer with 4F2hc for system xc- activity
"The latter protein, named xCT, showed a significant homology with those recently reported to mediate cationic or zwitterionic amino acid transport when co-expressed with 4F2hc"
Structure, function, and regulation of human cystine/glutamate transporter in retinal pigment epithelial cells.
Molecular cloning and expression of human xCT, the light chain of amino acid transport system xc-.
Identification and characterisation of human xCT that co-expresses, with 4F2 heavy chain, the amino acid transport activity system xc-.
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Demonstrated Na+-independent transport activity specific for L-cystine, L-glutamate and L-aspartate
"The amino acid transport activity induced by the co-expression of human 4F2hc and xCT in Xenopus oocytes was sodium independent and specific for L-cystine, L-glutamate and L-aspartate"
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Established 1:1 stoichiometry of cystine/glutamate exchange
"This activity also functioned in an exchange mode (e.g. cystine/glutamate) with a substrate stoichiometry of 1:1"
Membrane topology of system xc- light subunit reveals a re-entrant loop with substrate-restricted accessibility.
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Established 12-TM topology with intracellular N and C termini
"we propose a topological model for xCT of 12 transmembrane domains with the N and C termini located inside the cell"
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Identified re-entrant loop with substrate-restricted accessibility
"Studies of biotinylation and accessibility to sulfhydryl reagents revealed a re-entrant loop within intracellular loops 2 and 3"
Differential expression of intestinal membrane transporters in cholera patients.
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SLC7A11 upregulated in acute cholera patients as part of response to toxic substance
"Two amino acid transporters, SLC7A11 and SLC6A14, were upregulated in acute cholera patients compared to convalescence"
CD44 variant regulates redox status in cancer cells by stabilizing the xCT subunit of system xc(-) and thereby promotes tumor growth.
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CD44 variant interacts with xCT and controls intracellular GSH levels
"CD44 variant (CD44v) interacts with xCT, a glutamate-cystine transporter, and controls the intracellular level of reduced glutathione (GSH)"
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Ablation of CD44 induces loss of xCT from cell surface
"Ablation of CD44 induced loss of xCT from the cell surface and suppressed tumor growth in a transgenic mouse model of gastric cancer"
KSHV attachment and entry are dependent on αVβ3 integrin localized to specific cell surface microdomains and do not correlate with the presence of heparan sulfate.
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CD98 detected at cell surface microdomains
"the presence of CD98 and heparan sulfate (HS), the putative attachment receptor, was more variable"
An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
A reference map of the human binary protein interactome.
N-acetylcysteine, xCT and suppression of Maxi-chloride channel activity in human placenta.
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xCT mediates N-acetylcysteine uptake in placenta
"This study suggests that xCT mediates N-acetylcysteine uptake into the placenta"
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xCT localized to microvillous membrane of placental syncytiotrophoblast
"xCT, which was localised to the microvillous membrane of the placental syncytiotrophoblast"
Molecular basis for redox control by the human cystine/glutamate antiporter system xc().
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Cryo-EM structure of system xc- in apo and glutamate bound states
"Here we present the cryo-EM structure of system xc- in both the apo and glutamate bound states"
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xCT consists of 12 transmembrane helices adopting canonical APC superfamily fold
"The light subunit of human xc- transporter, xCT, (SLC7A11), consists of 12 transmembrane helices (TMs)"
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System xc- is a dedicated cystine-glutamate antiporter
"Under redox stress conditions mammalian cells use a specific system to increase cystine uptake to increase GSH synthesis, termed system xc− a dedicated cystine-glutamate antiporter"
Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.
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SLC7A11 imports kynurenine which propagates anti-ferroptotic signaling
"We show that this effect requires KYN export from IDO1-expressing cells, which is then available for non-IDO1-expressing cells via SLC7A11, the central transporter involved in ferroptosis suppression"
The structure of erastin-bound xCT-4F2hc complex reveals molecular mechanisms underlying erastin-induced ferroptosis.
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Erastin induces ferroptosis by inhibiting system xc-
"The small-molecule compound erastin induces ferroptosis via inhibiting the cystine-glutamate antiporter system xc–"
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xCT regulates ferroptosis in multiple pathophysiological processes
"Recent studies have shown that xCT (SLC7A11) regulates ferroptosis in liver fibrosis, cardiomyopathy, and numerous other pathophysiological processes"
Multimodal cell maps as a foundation for structural and functional genomics.
Galectin-13 reduces membrane localization of SLC7A11 for ferroptosis propagation.
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Galectin-13 inhibits plasma membrane localization of SLC7A11 to promote ferroptosis
"Galectin-13, which binds to CD44 and inhibits the plasma membrane localization of SLC7A11 in neighboring cells, thereby accelerating neighboring cell death and promoting ferroptosis propagation"
SLC7A11 is an unconventional H(+) transporter in lysosomes.
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SLC7A11 mediates slow lysosomal H+ leak through cystine/glutamate flux
"SLC7A11, the protein target of the ferroptosis-inducing compound erastin, mediates a slow lysosomal H+ leak through downward flux of cystine and glutamate"
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SLC7A11 deficiency causes lysosomal over-acidification and ferroptosis
"SLC7A11 deficiency or inhibition caused lysosomal over-acidification, reduced degradation, accumulation of storage materials, and ferroptosis"
The solute carrier superfamily interactome.
Basigin binds CD98 complex
SLC7A11-mediated exchange of extracellular cysteine and cytosolic glutamate
AcK-NFE2L2-dependent SLC7A11 expression
Deep research report on SLC7A11
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System xc− exchanges extracellular L-cystine for intracellular L-glutamate at ~1:1 stoichiometry to supply cysteine for GSH synthesis
"System xc− exchanges extracellular L-cystine for intracellular L-glutamate at approximately 1:1 stoichiometry, enabling cystine import that is reduced intracellularly to cysteine for glutathione (GSH) synthesis; reported Km values are ~50 µM (cystine) and ~200 µM (glutamate)"