Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism.
-
Purified P. aeruginosa LolCDE, reconstituted in proteoliposomes, releases lipoproteins to LolA in an ATP-dependent manner; LolB then incorporates them into the outer membrane.
"When incubated in the presence of ATP and a LolA homologue, the reconstituted LolCDE homologue released lipoproteins, leading to the formation of a LolA-lipoprotein complex."
-
P. aeruginosa LolCDE does not release lipoproteins carrying the Lys3-Ser4 inner-membrane retention signal.
"As revealed in vivo, lipoproteins with Lys and Ser at positions 3 and 4, respectively, remained in proteoliposomes."