GOA has no annotation for this protein, although it has been characterized experimentally since
2007. Its Pfam (AAA_31) and PANTHER (PTHR13696:SF52) matches bring no GO. NCBI names the WP_
protein "chromosome partitioning protein Soj" from BlastRule NBR012933, which carries no GO.
Experimental evidence (Jagura-Burdzy lab, strain PAO1161).
- Cytosolic, about 600 molecules per cell in exponential phase, falling about fivefold in
stationary phase. parA inactivation or overexpression gives anucleate cells and motility
changes, and parA loss destabilises ParB PMID:17545287. Abstract only in the cache.
- ATPase activity of purified ParA-His6; dimerization and ParB-interaction region E67-L85. The
parA84 allele (no dimerization, no ParB binding) phenocopies the null; parA67 (no ParB binding)
is milder [PMID:25139949 "ParA-His6 demonstrated ATPase activity (Fig. 3) of ~1.2 pmol Pi
released min−1 (pmol ParA)−1."; "Only PAO1161 parA84 was visibly impaired in terms of
chromosome segregation, growth rate and motility, similar to a parA-null mutant."].
- The parA and parB mutant transcriptomes overlap: a "Par regulon" of 536 genes PMID:24498062.
- Disrupting ParABS is very detrimental in P. aeruginosa PMID:23658532.
- DnaA regulates the gidAB-parAB operon from oriCII PMID:42156454.
Not proposed. DNA binding (inferred only from conserved Soj arginines, PMID:18077387) and ParB
stimulation of ATPase (shown in Myxococcus, PMID:40998818, not in P. aeruginosa). Both are listed
as suggested questions.
Decisions. NEW ATP hydrolysis activity (IDA), chromosome segregation (IMP), protein
homodimerization activity (IPI, two-hybrid only) and cytoplasm (IDA; the source abstract
says "cytosolic", but the method is not stated and ParA-family proteins are expected to
associate with the nucleoid, so the broader term is used).