Human reference: https://www.uniprot.org/uniprotkb/O15382/entry
Primary source: PMID:17050531 Human mitochondrial branched chain aminotransferase isozyme: structural role of the CXXC center in catalysis..
Mammalian branched chain aminotransferases (BCATs) have a unique CXXC center.
Kinetic and structural studies of three CXXC center mutants (C315A, C318A, and
C315A/C318A) of human mitochondrial (hBCATm) isozyme and the oxidized hBCATm
enzyme (hBCATm-Ox) have been used to elucidate the role of this center in hBCATm
catalysis. X-ray crystallography revealed that the CXXC motif, through its
network of hydrogen bonds, plays a crucial role in orienting the substrate
optimally for catalysis. In all structures, there were changes in the structure
of the beta-turn preceding the CXXC motif when compared with wild type protein.
The horse sequence comparison is in BCAT2-bioinformatics/RESULTS.md. Transfer is assessed for this exact accession; human biochemical evidence is not equine experimental validation.