Normal publication caches and separately recovered original passages retain distinct access states. Abstract-only flags are unchanged. Selected passage reading does not imply exhaustive supplementary-file or figure-pixel inspection.
| Source | Actual evidence read | Scope and limitation |
|---|---|---|
| 8626589 | Original PDF, Methods p.10238 and Results/Figure 3 pp.10239–10240 | Human endothelial cDNA/COS-7 assays; cDNA truncation removes untranslated sequence, not protein sequence. Normal cache abstract-only. |
| 19744926 | Original PDF, Methods pp.31064–31065, Results pp.31067–31071, Discussion pp.31071–31072 | Human full-length/engineered Δ58 and separate mouse knockout lipidomics; enriched lysates, no sn-position assignment. Normal cache abstract-only. |
| 21477596 | Full-cache selected Methods/Results, DGKE constructs, preparation and activity | Human FLAG-DGKE/mutants in COS-7 extracts; PIP5Kalpha is a separate enzyme. |
| 22108654 | Full-cache selected Methods/Results, substrate panel/Figure 1/Table 1 discussion | Human His6-DGKE in Sf21 extracts; sn-2 selectivity stronger than sn-1. |
| 18004883 | Author-repository original, Methods/Results pp.14227–14230, Table 1/Figure 5 | Human His6-DGKE in Sf21 extracts with mock/no-enzyme controls; possible substrate-isomer contamination. Normal cache abstract-only. |
| 23542698 | Methods/Results including Figure 4b | Endogenous human platelet membrane/cytoplasmic fractionation; rat and human tissue data distinguished; no unique membrane compartment. |
| 23274426 | Human constructs, knockdown and M3/TRPC6 Methods/Results | Receptor-driven currents are a DAG-sensitive readout, not direct DAG concentration. |
| 11287665 | Mouse cloning/knockout, electrophysiology and lipid-signaling Methods/Results | Perforant path–dentate granule synaptic potentiation; no direct protein localization. Normal cache abstract-only. |
| 15544348 | Complete abstract | Explicit epsilon experiment despite alpha-focused title; full Methods unavailable. |
| 23949095 | Complete abstract | Epsilon in ten-isozyme activity comparison; species/construct details unavailable. |
| 19946888 | Complete abstract and bibliographic identity | YTS membrane proteomics; target peptide/table evidence unavailable. Broad membrane annotation retained on curator deference and independent human platelet evidence; this particular target-table identification was not verified. |
The UniProt P52429 record and all-isoform sequences identify products of 567 and 177 residues. Sequence features were reconstructed independently. The natural short product is distinct from UTR-deleted cDNA and engineered Δ58 proteins. Retaining or losing a domain is not an experimental activity measurement.
The HPA subcellular record identifies cytosol/nucleoplasm staining. Only the existing cytosol assertion and its supported broad-term refinements are used. The PAINT reconstruction uses the actual PTHR11255 target lineage and root IBD PTN007499105; literal experimental donor records are distinguished from inferred transfer.
Reactome R-HSA-426240 represents DAG phosphorylation. Catalyst 426239 → set 426070 → DGKE member 426061 identifies human P52429 residues 1–567 at plasma membrane. The platelet pathway R-HSA-76002 reaches that reaction through DAG/IP3 signaling 114508. This establishes the curated pathway role but not an independently localized native organelle or a selective platelet-activation mechanism.
Short exact publication anchors occur once each across YAML and notes. The four-paper original locators and assay/product limits are described in the review notes. There is one catalytic core and no new annotation; general lipid-phosphorylation and DAG-metabolism annotations describe its reaction rather than separate biochemical activities.