quiP (PA1032, Q9I4U2) review notes

Review session 2026-11-05 (PseudoCAP IBA-seed protocol, tier 2)

Fetched with just fetch-gene PSEAE Q9I4U2 --alias quiP (first attempt hit a UniProt 429);
accession matches the worklist. Reviewed entry. No deep-research provider was used; literature
from the cached seed paper (full text). Reviewed together with pvdQ and PA0305 (shared node
PTN002151232; node-level record kept in genes/PSEAE/pvdQ/pvdQ-notes.md).

Literature

PMID:16461666 (Huang et al. 2006), full text cached and read.
Mutant: PMID:16461666, complemented by plasmid quiP.
Activity: PMID:16461666 E. coli expressing quiP released near-stoichiometric HSL from C8-HSL and
degraded C7 to C14 acyl-HSLs, not C4/C6.
Location is predicted only: PMID:16461666.
The PseudoCAP row is coded IMP, but the decisive data are the heterologous-expression
activity assay (whole cells, product HSL measured by LC/MS), which is closer to IDA. The
evidence code understates the support; not worth a defect.

Annotation decisions

GO:0016811 (IBA, IMP, IEA): ACCEPT, core MF (no GO term for AHL acylase; one is proposed on
the pvdQ review).
GO:0016787 (IEA): TOO_GENERAL.
GO:0017000 antibiotic biosynthetic process (IEA, InterPro S45): REMOVE (penicillin acylase
mapping; QuiP is an AHL acylase).
GO:0042597 periplasmic space (IEA): ACCEPT (signal peptide 1-26; family).
Action counts: ACCEPT 4, TOO_GENERAL 1, REMOVE 1.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002151232	GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	3	21 (13 taxa)	GO:0016811, IMP, PMID:16461666 (PseudoCAP)	SOUND	Heterologous expression confers long-chain AHL acylase activity with HSL product measured (full text); mutant cannot grow on C10-HSL.	pvdQ Q9I194 (purified enzyme, IDA), PA0305 Q9I6I6 (purified enzyme, IDA)	Yes (see pvdQ notes for the node-level record)

No PSEUDOCAP or GO_CENTRAL defect on quiP.