file:PSEPK/cheY/cheY-deep-research-falcon.md

Deep research report on cheY (PP_4340) in Pseudomonas putida KT2440

Synthesises KT2440 genomic context, the KT2440 CheY-CheA crosslinking study and general CheY mechanism.

"A KT2440 transcriptomic study of a **fliA** mutant (σ factor for flagellar genes) identifies **PP_4340** explicitly as “Chemotaxis protein CheY”"

file:PSEPK/cheY/cheY-uniprot.txt

UniProt entry Q88EW2 for Pseudomonas putida KT2440 CheY

PROSITE assigns a response regulatory (REC) domain over residues 2-119 and a 4-aspartylphosphate at Asp52. The cofactor and subcellular location lines rest on ARBA rules only.

Gene Ontology annotation through association of InterPro records with GO terms

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

PMID:40197389

Coordinated regulation of chemotaxis and resistance to copper by CsoR in Pseudomonas putida.

Purified P. putida CheA transfers its phosphoryl group to CheY in vitro; CheY co-purified with CheA from KT2440 lysate.

"the response regulator CheY and the phosphatase CheZ (two proteins known to be associated with CheA) also showed high Log2(fold change) values"

PMID:30761113

Concentration Dependent Effect of Plant Root Exudates on the Chemosensory Systems of Pseudomonas putida KT2440.

cheA2 (PP_4338), encoded beside cheY, is required for chemotaxis to maize root exudates.

PMID:36287825

Genetic Code Expansion in Pseudomonas putida KT2440

KT2440 CheY was used as a photocrosslinking target for protein-protein interaction studies; the deep-research report states that crosslinking proteomics recovered CheA.

PMID:25100612

Pseudomonas chemotaxis

Review of Pseudomonas chemotaxis pathways and assays; background for the canonical CheA-CheY-CheZ scheme.