Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.
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TAP purification with DALPC mass spectrometry defined a 13-subunit APC/C in both S. pombe and S. cerevisiae; Apc2 is among the recovered fission yeast subunits.
"Our data increase the total number of identified APC subunits to 13 in both yeasts and indicate that previous approaches were biased against the identification of small subunits."
Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
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Cut9-TAP and Apc13-TAP purifications from fission yeast recover the same set of APC/C subunits.
"purification of a TAP-tagged version of Apc13 yielded the same set of APC/C subunits as Cut9-TAP"
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Apc11 carries the RING finger and is anchored to the complex through the cullin-related subunit Apc2.
"Apc11 is anchored to the complex through Apc2, a subunit distantly related to cullins"
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Proteome-wide YFP-tagging localisation atlas for S. pombe; source of the HDA nucleus and cytosol rows.
"Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein."
The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27.
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Apc2 and Apc11 form the catalytic centre of the APC/C, analogous to Cul1-Rbx1 of the SCF.
"The catalytic centre of the APC/C is formed from the cullin and RING subunits Apc2 and Apc11, analogous to Cul1 and Rbx1, respectively, of the SCF cullin RING ligase."
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The N-terminal region of Apc2 consists of three cullin repeats.
"the N-terminus of Apc2 comprises three consecutive ∼130 residue cullin repeats"
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Apc1 bridges the Apc2-Apc11-Apc10 catalytic subcomplex to the TPR subunits.
"Apc1 functions as a central scaffolding subunit that links the catalytic subcomplex formed from Apc2, Apc11 and Apc10/Doc1 with the TPR subunits Cdc16/Cut9, Cdc23 and Cdc27 via Apc4 and Apc5."
Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast.
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The fission yeast 20S APC/cyclosome carries the ubiquitin ligase activity required for cyclin and Cut2/securin destruction.
"We show here that the fission yeast gene products Cut9 and Nuc2 are the subunits of the 20S complex, the putative APC (anaphase promoting complex)/cyclosome which contains ubiquitin ligase activity required for cyclin and Cut2 destruction."
APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex.
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A human APC2-APC11 heterodimer with Ubc4 or UbcH10 ubiquitinates securin and cyclin B1 but lacks destruction-box specificity.
"However, the minimal APC2/11 ubiquitin ligase module does not possess substrate specificity, because it also ubiquitinates the destruction box deletion mutants of securin and cyclin B1."
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APC11 and UbcH10 bind the C-terminal cullin homology domain of APC2.
"Both APC11 and UbcH10 bind to the C-terminal cullin homology domain of APC2, whereas Ubc4 interacts with APC11 directly."
The APC11 RING-H2 finger mediates E2-dependent ubiquitination.
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Budding yeast Apc11 alone mediates E2-dependent ubiquitin transfer in vitro; Apc2 is proposed to tether and position Apc11 within the complex.
"While Apc2p was not required to effect ubiquitin transfer in vitro, it may function in a cellular context to tether Apc11p to APC core proteins, and to properly position the Apc11p RING-H2 finger with respect to its substrates."
Identification of a physiological E2 module for the human anaphase-promoting complex.
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The budding yeast APC/C uses Ubc4 to initiate and Ubc1 to elongate K48-linked chains, whereas the human complex uses UbcH10 and Ube2S to build K11-linked chains.
"However, Ubc4 and Ubc1 function sequentially to assemble K48-linked ubiquitin chains, whereas human UbcH10 and Ube2S most likely bind APC/C at the same time."
UniProtKB record for S. pombe apc2 (Q874R3)
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Apc2 belongs to the cullin family and is one of the 13 subunits of the fission yeast APC/C.
"Belongs to the cullin family."
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Subunit composition of the S. pombe APC/C.
"The APC/C is composed of at least 13 subunits: apc1, apc2,"
Deep research summary for S. pombe apc2 (Edison/falcon)
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Apc2 was recovered from two independent S. pombe APC/C purifications with 19 and 24 unique peptides.
"Apc2 yielded 19 unique tryptic peptides in a Lid1-TAP preparation and 24 in an independent Apc13-TAP preparation."
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Apc2 is present at one copy per APC/C particle.
"differently tagged Apc2 molecules did not self-coimmunoprecipitate"
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The S. pombe cryo-EM map places the Apc2-Apc11-Apc10 catalytic region next to the Slp1 coactivator.
"Mapping placed the Apc2–Apc11–Apc10 catalytic region along the right/lower side of the complex, with Apc11 near the cavity lip and Slp1 near the lower horn-like region."
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Apc2 supplies the cullin scaffold while Apc11 supplies the RING.
"Apc11 supplies the RING element that activates or positions the E2–ubiquitin conjugate; Apc2 supplies the cullin-like scaffold."
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The fission yeast APC/C uses the E2s Ubc4 and Ubc11 for cyclin B ubiquitylation.
"In fission yeast, Ubc4 and Ubc11 contribute to different aspects of cyclin-B ubiquitylation."
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APC/C-driven securin and cyclin B destruction drive sister-chromatid separation and mitotic exit.
"Securin destruction activates separase and sister-chromatid separation; cyclin-B destruction lowers Cdk activity and permits mitotic exit."