Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Dictyostelium kinase DPYK3 negatively regulates STATc signaling in cell fate decision.
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dpyk3- cells show aberrant prestalk/prespore patterning and persistent DIF-1-induced STATc phosphorylation, rescued by DPYK3 re-expression.
"dpyk3- cells displayed persistent STATc phosphorylation"
Identification of the protein kinases Pyk3 and Phg2 as regulators of the STATc-mediated response to hyperosmolarity.
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pyk3- cells have reduced stress-induced STATc phosphorylation, delayed STATc nuclear translocation and reduced induction of STATc target genes; Pyk3 is not the PTP3 serine kinase.
"Pyk3 and Phg2 are required for full transcriptional activation of STATc-dependent genes."
Two Dictyostelium tyrosine kinase-like kinases function in parallel, stress-induced STAT activation pathways.
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Pyk3 autophosphorylates on tyrosine and directly phosphorylates STATc on Y922; its pseudokinase domain inhibits the TKL domain.
"In the construct GST-Pyk3-KI, where KI was expressed in isolation from KII, the TKL directed tyrosine phosphorylation of His-STATc on Y922."
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Pyk3 moves from the cytosol to the F-actin-rich cortex upon hyperosmotic stress.
"Before sorbitol treatment, Pyk3 is also enriched in the cytosol, but, after exposure to sorbitol, the amount at the cortex increases, where it often colocalizes with F-actin"
Classification of tyrosine kinases from Dictyostelium discoideum with two distinct, complete or incomplete catalytic domains.
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DPYK3 C-terminal fragment autophosphorylates on tyrosine; protein has two tandem kinase-related domains.
"A common feature of these kinases is the presence of two different sequence stretches in tandem that are related to kinase catalytic domains."