RNR1 (YER070W, P21524) notes

Review context: module dntp_de_novo_synthesis (class Ia RNR large subunit; EC 1.17.4.1; ADP/GDP/CDP/UDP reduction).

Evidence journal

• R1 carries active site and effector sites PMID:10716984; holoenzyme alpha2-beta-beta' with Rnr2/Rnr4 PMID:10716984.
• Recombinant Y1 activity PMID:10535923.
• Rnr3 has <1% of Rnr1 activity PMID:11893751.
• Cytoplasmic; stays cytoplasmic after damage, small subunits move PMID:12732713; PMID:18851834.
• Structures with substrates/effectors PMID:16537479; alpha6 hexamer PMID:21336276; C-terminal CX2C regenerates neighbouring active site PMID:17277086; Sml1 allosteric inhibitor PMID:27155231.
• Multicopy RNR1 rescues mip1 (mtDNA polymerase) mutants PMID:8552025.

Curation observations

• YeastPathways RCA GO:0106387 'de novo' GMP biosynthetic process (from PWY-7222-1) is a frame mis-mapping: REMOVE.
• Nuclear HDA (PMID:22842922) conflicts with dedicated localization studies: MARK_AS_OVER_ANNOTATED.
• 11 protein-binding IPI rows removed per policy (Rnr2, Rnr4, Sml1 partners); holoenzyme captured by GO:0005971.