Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
Identification of the mitochondrial glutamate transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution of two human isoforms.
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GC-1 (SLC25A22) and GC-2 (SLC25A18) are glutamate/H+ symporters of the mitochondrial inner membrane; the bacterially expressed proteins were reconstituted into liposomes and shown to co-transport glutamate with a proton (or in exchange for OH-).
"Glutamate is co-transported with H(+) (or exchanged for OH(-))"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Impaired mitochondrial glutamate transport in autosomal recessive neonatal myoclonic epilepsy.
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The DEE3/EIEE3 disease variant Pro206Leu in SLC25A22 disrupts L-glutamate transporter activity, establishing loss of mitochondrial glutamate/H+ transport as the disease mechanism.
"P -> L (in DEE3; disrupts L-glutamate transporter"
SLC-mediated transport of neurotransmitters
SLC25A18,22 import L-Glu, H+
Malate-aspartate shuttle
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In the malate-aspartate shuttle, glutamate is coimported with a proton (the glutamate/H+ import leg to which SLC25A22 contributes) and transaminated in the matrix.
"which subsequently gets coimported with a proton and transaminated back"