FKTN source guide

This guide records the evidence access used for the FKTN review. Normal publication, UniProt, QuickGO, Reactome and family files were obtained through their standard routes and were not hand-edited. Separately recovered originals remain distinct from abstract-only normal caches.

Original sources and independent readings

• PMID:26923585 original Kobe PDF: original Results/Discussion and enzyme Methods read in the independent ROOT consultation; selected pages 8,12,13 visually inspected there. SHA256: 811ef34a76ad1513e6d267b08b0bbb66462316a68f8c85a4cd0586e0dc69102c. NMR establishes GalNAc-C3 attachment; separate pNp acceptors lack mannose phosphate. Insert species and uninspected supplement boundaries remain explicit.
• PMID:27194101 full primary: mouse enzymes, rabbit dystroglycan and human host cells distinguished; donor transfer and rescue do not independently resolve every linkage.
• PMID:25279699 publisher full text: human FKTN pTW322 versus mouse Fkrp pTW323; human patient fibroblasts are separate experiments.
• PMID:27601598 full primary: selected Methods/Results and Figures 6–7 in the association consultation. Co-recovery and HCT116/HEK293T glycoforms remain distinct; alternative donor models are hypotheses.
• PMID:29477842 abstract plus author-deposited doctoral summary: the summary is supplementary primary evidence, not journal full Methods. Tagged triple co-IP, GM130 localization, activities and FKTN product MS were described; endogenous FKTN was detected, endogenous FKRP was not independently visualized.
• PMID:29884773 full primary: FKTN/FKRP donor competition and soluble enzyme assays. FKTN kinetic parameters are not reassigned to FKRP; no physiological glycerol-regulation annotation is manufactured.
• PMID:18808525 full primary and PMID:34830034 full primary: actual selected Methods/Results/Discussion read, preserving cancer-cell identity, doses, timepoints, controls and unresolved mechanistic differences. No figure-pixel or supplement audit is claimed for these readings.
• PMID:17034757(https://pubmed.ncbi.nlm.nih.gov/17034757/) and official IntAct: full publisher Methods unavailable; abstract and curated assay records support association/localization with stated limits. Exact human pair O75072/Q8WZA1, tagged COS-7 and one C2C12 colocalization context were recovered.
• PMID:15213246(https://pubmed.ncbi.nlm.nih.gov/15213246/): the normal cache is abstract-only; original scanned pages 709–712 were independently read in the localization consultation. FKTN is the transfected myc cis-Golgi comparator; FKRP endogenous rough-ER observations are not evidence for FKTN ER localization.
• PMID:9690476(https://pubmed.ncbi.nlm.nih.gov/9690476/): original abstract and metadata support historical disease/secretion contexts. Detailed construct and endogenous secretion assays remain unavailable.
• BioPlex PMID:28514442(https://pubmed.ncbi.nlm.nih.gov/28514442/) and PMID:33961781(https://pubmed.ncbi.nlm.nih.gov/33961781/): curated interaction contexts plus independent targeted corroboration. Exact supplemental edge, bait direction, cell-line allocation and spectra were not replayed; the 33961781 normal full-text flag does not imply Methods access.

Ontology and phylogeny

GO:0016780 covers substituted-phosphate transfer. GO:0047356 describes ribitol-phosphate-polymer extension; the name does not by itself justify replacement for mammalian glycopeptide transfer, and no hard bacterial taxon restriction is asserted. GO:0035269 includes later extension of an O-mannose glycan. GO:1902494 requires a catalytically active complex; membership here integrates recovery with activity evidence.

Official GO path confirms GO:0000139 part_of GO:0005794 part_of GO:0005737. The exact 188-byte response SHA256: a37f7f3aea05c7f1900929078d604158bfee3b1262ea57e4830c75d7fddede42 supports the two TOO_GENERAL cytoplasm decisions. This is a precision judgment, not a false-location claim.

The original PAINT IBD assertions at PTN001034988 and PTN000395622 were matched to the human FKTN target path in the complete 115-node PTHR15407 topology. Original IBD, official family topology, normal family cache and incoming QuickGO provenance were distinguished. Human FKTN among experimental seed evidence is not circular; fungal descendants do not negate an ancestral glycan-biosynthesis assertion. The normal PTHR15407 entries, metadata and paint files were preserved byte-for-byte, without bulk leaf-GAF download.

Private reproducible PAINT proof SHA256: 2c31a1b9925e962049d9bf0b6dfac012bd114f685d58809c5992e16d0e33e0d4. Original QuickGO 44-context response SHA256: 20d79b929cc5a3938ba01fc88c3d11f0d89890fdd739401bcabd374e62ef2c74. The complete context mapping is described in source evidence.

Normal-source conservation

Direct source receipts for the independent replay: complete PTHR15407 tree, retrieved 2026-10-28T16:09:58Z, SHA256: 602c3a4e8bc859c0ba859c3b6373257213b7b9bfbc0b7e68e11c5613dea91a30; original IBD.gaf, retrieved 2026-10-28T05:21:02Z, last-modified 2026-08-28, SHA256: 0c672914e10c1f79d3fb365077d843a92e518eb6eee2aac00c67150274adf569; original 44-context QuickGO response, retrieved 2026-10-28T15:25:45Z, SHA256: 20d79b929cc5a3938ba01fc88c3d11f0d89890fdd739401bcabd374e62ef2c74. Separate retrieval provenance does not assert whole-release synchronization.

The original scanned PMID:15213246 article has SHA256: 65d858e4d7683851fca71242c712a0814c7600699d7d3bdc36b0ecef3ab7fd0e; journal pages 709–712 correspond to PDF pages 7–10. The independent localization consultation read those pages visually. This does not imply that figure pixels or supplements from other sources were inspected.

Seventeen sources were present on qualified main d50d8c566c7a9ddc56c56e226483608c0717faef and were hydrated/verified before fetch. Four absent sources (PMID:9690476,17034757,18808525,34830034) were acquired normally. All 21 final normal-source identities are below; no provider-generated research text was authored.

Follow-up evidence interpretation

The official mouse Fktn donor record Q8R507 establishes donor identity. Its presence in the original source field is preserved; no claim of an independently replayed donor localization experiment is made. The nuclear judgment uses the already-read human experiments linked above.

For the proliferation comparison, PMID:18808525 Results/Figure 6 and Methods report increased Ki-67 labeling after 20 nM F697 at five days in HeLa and ZR-75-1. PMID:34830034 Results/Figure 2 and RNAi Methods report reduced viable-cell density/Ki-67 after 40 nM F697 at four days in 1321N1, with increased readouts after overexpression. Its Results/Figure 7 report unchanged JNK/c-Jun phosphorylation ratios. These are separate observations; both measured proliferation findings remain CURRENT, with the differing directions and limits recorded in finding_review.review_notes and reference-only support. No hypothetical general claim is attributed to the earlier authors as a DISPUTED finding; no superseded_by link or unmatched consistency_exception is added. The JNK annotation is over-specific because the earlier experiment did not isolate JNK regulation from its downstream phospho-c-Jun readout. Captions and text were read; no new figure-pixel or supplement audit is claimed.

Broader glycoprotein and O-linked-glycosylation process annotations remain informative pathway contexts beside the precise O-mannose-glycan extension step. This follows the annotation-reviewer distinction between a useful pathway-level process and a location term that adds no precision. The original ER immunogold ambiguity remains unresolved and is not repaired by assuming normal biosynthetic transit.