PMID:10398681

Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana.

The complex comprises TIR1, ASK and AtCUL1.

"The complex consists of proteins related to yeast Skp1p and Cdc53p called ASK and AtCUL1, respectively, as well as the F-box protein TIR1."

PMID:11019805

Protein degradation in signaling.

Provides historical context for proteolytic signaling rather than target-specific catalytic evidence.

PMID:11713520

Auxin regulates SCF(TIR1)-dependent degradation of AUX/IAA proteins.

SCF(TIR1) is required for Aux/IAA degradation.

"SCF(TIR1) is required for AUX/IAA degradation"

PMID:15917797

The F-box protein TIR1 is an auxin receptor.

TIR1 is directly identified as an auxin receptor.

"TIR1 is an auxin receptor that mediates Aux/IAA degradation"

PMID:15917798

The Arabidopsis F-box protein TIR1 is an auxin receptor.

Independently supports TIR1 receptor identity.

PMID:17410169

Mechanism of auxin perception by the TIR1 ubiquitin ligase.

InsP6 is an integral structural cofactor in the TIR1 LRR domain.

"the leucine-rich repeat domain of TIR1 contains an unexpected inositol hexakisphosphate co-factor"

PMID:18391211

Small-molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling.

Crystallography verifies small-molecule binding to TIR1.

"their mode of action in binding to TIR1 is confirmed by x-ray crystallographic analysis"

PMID:18628351

Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation.

Receptor combinations change pollen maturation and anther dehiscence timing.

"in tir1 afb triple and quadruple mutants, anther dehiscence and pollen maturation occur earlier than in the wild type"

PMID:19106375

Phosphate availability alters lateral root development in Arabidopsis by modulating auxin sensitivity via a mechanism involving the TIR1 auxin receptor.

Root phosphate-starvation responses recruit TIR1-dependent auxin sensitivity.

PMID:26236497

Untethering the TIR1 auxin receptor from the SCF complex increases its stability and inhibits auxin response.

SCF association controls receptor stability and auxin response.

PMID:9436980

The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast grr1p.

tir1 mutants impair lateral-root formation.

"The tir1 mutants are deficient in a variety of auxin-regulated growth processes including hypocotyl elongation and lateral root formation."

GO_REF:0000033

Annotation inferences using phylogenetic trees

file:ARATH/TIR1/TIR1-uniprot.txt

UniProt record for Arabidopsis TIR1

TIR1 is described as an auxin receptor and SCF(TIR1) component.

file:ARATH/TIR1/TIR1-deep-research-falcon.md

Falcon deep research report for Arabidopsis TIR1

Falcon research supports TIR1 as an F-box/LRR auxin receptor and SCF substrate adaptor for Aux/IAA degradation.

file:interpro/panther/PTHR16134/PTHR16134-entries.csv

PANTHER PTHR16134 entries

TIR1 and COI1 are in distinct F-box/LRR subfamilies, avoiding auxin/jasmonate receptor conflation.

PMID:36289340

Adenylate cyclase activity of TIR1/AFB auxin receptors in plants.

Purified TIR1 has adenylate cyclase activity; the author manuscript supplies construct-specific controls.

file:publications/url_https___pure.coventry.ac.uk_ws_portalfiles_portal_60931587_Post_Print.pdf.md

Adenylate cyclase activity of TIR1/AFB auxin receptors in plants (author-accepted manuscript)

Purified full-length TIR1 has a measured cAMP-production rate.

"Vmax = 7.462 fmol/min/µg and KM = 0.644 mM for TIR1"

PMID:40044868

TIR1-produced cAMP as a second messenger in transcriptional auxin signalling.

AC mutations preserve the TIR1–ASK1 interaction.

"TIR1ACm1 and TIR1ACm3 do not affect the TIR1 interaction with ASK1"
The tested AC mutants separate cAMP production from Aux/IAA degradation.

"TIR1/AFBs AC and SCFTIR1 E3 ligase activities are independent"
Full-length IAA6/IAA7/IAA8/IAA17 reporter degradation is preserved across control and AC-mutant ccvTIR1 lines.

"comparable cvxIAA-induced degradation of all these Aux/IAAs in pTIR1::ccvTIR1, pTIR1::ccvTIR1ACm1 and pTIR1::ccvTIR1ACm3"
The matched AC-mutant lines reduce or eliminate DR5 reporter activation.

"cvxIAA-induced DR5::Luc activation was strongly reduced in pTIR1::ccvTIR1ACm1 and completely lost in the pTIR1::ccvTIR1ACm3 line"

file:interpro/panther/PTHR16134/PTHR16134-paint.tsv

PANTHER PTHR16134 PAINT ancestral assertions

Distinct ancestral nodes encode receptor versus general SCF functions.