Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Correlation between catalytic activity and monomer-dimer equilibrium of bacterial alanine racemases.
-
Seven biosynthetic and catabolic alanine racemases, including those of P. aeruginosa, are monomer-dimer equilibria; high Vmax correlates with dimerisation.
"In both biosynthetic and catabolic alanine racemases, association constants for dimerization were high for the enzymes with high V(max) values."
Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1.
-
alr and dadX overexpressed, complement E. coli D-alanine auxotrophs, purified and characterised.
"The dadX(PA) and alr(PA) genes have been sequenced, overexpressed, and their activity was demonstrated by complementing d-alanine auxotrophs of Escherichia coli."
Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.
-
Crystal structure (PDB 6A2F) and biochemical characterisation of P. aeruginosa Alr; conserved homodimer.
"Here we report the crystal structure of biosynthetic alanine racemase (Alr) from a pathogenic bacteria Pseudomonas aeruginosa PAO1."
UniProt entry Q9HUN4 (ALR1_PSEAE)