Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Automated transfer of experimentally-verified manual GO annotation data to mouse-human orthologs
Combined Automated Annotation using Multiple IEA Methods
Characterization of mouse angiogenin-related protein: implications for functional studies on angiogenin.
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Mouse Ang2/Angrp is not angiogenic in assays where mouse angiogenin is active.
"mouse Ang is potently angiogenic, but Angrp is not"
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Mouse Ang2/Angrp retains ribonucleolytic activity toward tRNA and dinucleotide substrates.
"Angrp has somewhat greater ribonucleolytic activity toward tRNA and dinucleotide substrates than does Ang"
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Loss of angiogenic activity is attributed to defective cellular receptor binding rather than loss of RNase catalytic capacity.
"an inability to bind cellular receptors is implicated since Angrp does not inhibit Ang-induced angiogenesis"
Expression and regulation of murine macrophage angiopoietin-2.
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This UniProt tissue-specificity citation is about angiopoietin-2, not angiogenin-2/Angrp.
"Expression and regulation of murine macrophage angiopoietin-2."
High glucose increases angiopoietin-2 transcription in microvascular endothelial cells through methylglyoxal modification of mSin3A.
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This publication studies angiopoietin-2/Ang-2 expression, not mouse angiogenin-2/Angrp.
"High glucose increases angiopoietin-2 transcription in microvascular endothelial cells"
Crystal structures of murine angiogenin-2 and -3-probing 'structure--function' relationships amongst angiogenin homologues.
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mAng-2 has substantial tRNA-cleavage activity in comparative RNase assays.
"mAng-2 is almost 80% active compared to the human form of the protein"
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The mAng-2 crystal structure captured zinc bound at the active site.
"first crystal structures of an Ang with a zinc ion bound at the active site"
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The structural study reinforces that mAng-2 is not angiogenic despite RNase activity.
"whereas mAng-2 is not angiogenic"
Angiotensin II signaling via protein kinase C phosphorylates Kelch-like 3, preventing WNK4 degradation.
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This publication concerns angiotensin II regulation of KLHL3/WNK4, not the Ang2 gene product.
"Angiotensin II signaling via protein kinase C phosphorylates Kelch-like 3, preventing WNK4 degradation"
Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4.
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This publication concerns WNK4 phosphorylation downstream of AngII/vasopressin signaling, not the Ang2 gene product.
"With-no-lysine kinase 4 (WNK4) regulates electrolyte homeostasis and blood pressure"
Zinc-finger protein 418 overexpression protects against cardiac hypertrophy and fibrosis.
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This publication concerns ZNF418 and uses angiotensin II as a stimulus; it is not an Ang2/Angrp study.
"effect and mechanism of zinc-finger protein 418 (ZNF418) on cardiac hypertrophy caused by aortic banding"
Ang2 ISO source trace results
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All 46 ISO annotations in the local Ang2 GOA were transferred from human ANG through GO_REF:0000119.
"Every one of those 46 ISO rows is transferred through `GO_REF:0000119` from the same source entity, `UniProtKB:P03950` (human angiogenin/ANG)."
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Mouse Ang2 is a divergent paralog, so human ANG angiogenic, receptor/signaling, nuclear/stress, and immune annotations should not be propagated automatically.
"even ISO terms with real experimental support on human ANG are unsafe to accept automatically for mouse Ang2 when they concern angiogenesis, receptor binding, signaling, stress-granule biology, nuclear trafficking, or immune effector roles."
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The experimental GOA block contains homonym errors from angiopoietin-2, angiotensin II, and ZNF418 papers.
"The non-ISO experimental block in the local GOA file also contains clear homonym errors"
Falcon deep research report for mouse Ang2
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Falcon verified that mouse Ang2 is angiogenin-2/Angrp rather than angiopoietin-2.
"The UniProt target provided (Q64438) is instead a **murine angiogenin paralog**, commonly referred to as **mouse angiogenin-2 (mAng2)** or **angiogenin-related protein (Angrp)**"
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Falcon identified the best-supported direct function as secreted RNase activity with tRNA/dinucleotide substrates and little or no angiogenic activity.
"Best-supported annotation for Q64438 is: **secreted RNase A-family paralog with strong in vitro RNase/tRNA-cleaving activity but little or no angiogenic activity**"
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Falcon found no recent primary studies centered directly on mouse Ang2/Angrp.
"**Key limitation:** no 2023–2024 primary studies explicitly centered on **mouse Ang2/Angrp** were retrieved here."