Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase.
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StlB (Steely2) is a hybrid enzyme with six type I FAS-like catalytic domains fused to a C-terminal iterative type III (chalcone-synthase-like) PKS domain.
"Steely proteins possess six catalytic domains homologous to metazoan type I fatty acid synthases (FASs) but feature an iterative type III polyketide synthase (PKS)"
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The StlB type III PKS domain synthesizes PCP, the acylphloroglucinol skeleton of DIF-1 and its degradation product DIF-3.
"this type III PKS efficiently synthesizes PCP"
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StlB is the DIF-pathway PCP (THPH) synthase; the Steely2 knockout does not accumulate DIF-1 and is rescued by exogenous PCP.
"confirms Steely2-mediated catalysis of PCP biosynthesis in vivo"
DIF-1 induces the basal disc of the Dictyostelium fruiting body.
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The stlB (PKS) null mutant makes no DIF-1 and produces long, thin slugs, ~35% fewer prestalk cells, and fruiting bodies lacking the basal disc and a proper lower cup; defects are rescued by exogenous DIF-1.
"DIF-1 is required for slug migration and crucially for the formation of the lower cup and basal disc of the fruiting body"
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The DIF-1 backbone is a 12-carbon polyketide made by the steely B (stlB) PKS.
"The backbone of DIF-1 consists of a 12-carbon polyketide produced by the steely B (stlB) PKS"
Steely enzymes are involved in prestalk and prespore pattern formation.
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DIF-1 (product of SteelyB/StlB) is the major stalk cell inducer, and the stlA/stlB double knockout loses pstA marker gene expression.
"the stlA and stlB double-knockout mutant lost pstA marker gene expression"
c-di-GMP induction of Dictyostelium cell death requires the polyketide DIF-1.
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stlB disruption almost completely prevents c-di-GMP-induced vacuolar cell death, showing an stlB-dependent polyketide (DIF-1) is required.
"the stlB-initiated biosynthetic cascade included at least one polyketide required for the major part of cell death induction by c-di-GMP"
Stalk cell differentiation without polyketides in the cellular slime mold.
Yellow polyketide pigment suppresses premature hatching in social amoeba.