grim apoptosis curation notes

2026-09-30

• Reviewed all 23 seeded FlyBase GOA rows for grim using the local UniProt record and cached publications.
• Treated Grim as an RHG/IAP antagonist with two separable pro-apoptotic regions:
  an N-terminal IAP-binding motif that binds IAP BIR domains and a GH3 amphipathic
  helix that supports mitochondrial apoptotic changes.
• Downcasted generic GO:0006915 apoptotic process and GO:0012501 programmed cell death rows to GO:0097190 apoptotic signaling pathway where the source
  assayed Grim as the upstream pro-death trigger.
• Replaced the generic DIAP2 GO:0005515 protein binding row with
  GO:1990525 BIR domain binding; Ribeiro et al. directly state that Rpr and
  Grim preferentially bind the BIR2 domain of DIAP2 PMID:18166655.
• Broadened the GO:0005759 mitochondrial matrix row to
  GO:0005739 mitochondrion; Claveria et al. used a matrix marker to show that
  Grim-positive rings are mitochondria associated, not that Grim enters the
  matrix PMID:12093734.
• Broadened positive regulation of release of cytochrome c from mitochondria
  to GO:0008637 apoptotic mitochondrial changes because the same paper reports
  no substantial free cytochrome-c release into the cytosol in Grim-expressing
  Drosophila cells, only cytochrome-c redistribution into Grim-positive puncta
  PMID:12093734.
• Kept Malpighian tubule nuclear localization and neuronal/glial developmental
  death rows as non-core or context-specific: they are real deployments or
  restraint contexts of the Grim program rather than separate molecular
  activities.
• Left rows from abstract-only papers UNDECIDED when the visible cache did not
  expose Grim-specific evidence, notably lifespan, melanization, larval CNS
  remodeling, plasma membrane, and one Sickle-paper top-level apoptosis row.