Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Deep research report on TRUB2 in Columba livia (falcon/Edison)
-
TRUB2 is a mitochondrial pseudouridine synthase that catalyzes Psi55 formation in mitochondrial tRNAs, localizes to mitochondrial RNA granules, and is essential for mitochondrial translation and OXPHOS complex assembly.
"catalyzing the formation of pseudouridine at position 55 in the TΨC loop of select mitochondrial tRNAs"
A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.
-
Human TRUB2 is a mitochondrial RNA-granule pseudouridine synthase that, with RPUSD3, pseudouridylates specific residues in mitochondrial mRNAs; its depletion impairs mitochondrial protein synthesis without altering mt-mRNA levels.
"TRUB2/RPUSD3 were similarly involved in pseudouridylating specific residues in mitochondrial mRNAs"
Mammalian nuclear TRUB1, mitochondrial TRUB2, and cytoplasmic PUS10 produce conserved pseudouridine 55 in different sets of tRNA.
-
Recombinant human TRUB2 and mitochondrial extracts show tRNA Psi55 synthase activity, with stricter tRNA structural requirements than TRUB1.
"while TRUB1, PUS10, and TRUB2 all have tRNA Ψ55 synthase activities, they have different tRNA structural requirements"
UniProt entry A0A2I0M3K7 (Columba livia TRUB2 gene model)
Sequence completeness and evolutionary placement of A0A2I0M3K7
-
The pigeon entry is an N-terminally truncated TRUB2 gene model covering human TRUB2 residues 178-307 and lacking the catalytic aspartate.
"The catalytic aspartate position (human D98) lies outside the region covered by the pigeon sequence, so A0A2I0M3K7 lacks the catalytic aspartate."