Identification of an HptB-mediated multi-step phosphorelay in Pseudomonas aeruginosa PAO1.
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PA1611 transfers phosphoryl groups through HptB to the response regulator PA3346; the pathway affects flagellar activity, chemotaxis, twitching and biofilm formation.
"We herein demonstrate that the hybrid sensor PA1611 carries out specific signal transduction, through HptB (PA3345), to the response regulator PA3346 in Pseudomonas aeruginosa PAO1"
Structural insights into the histidine-containing phospho-transfer protein and receiver domain of sensor histidine kinase suggest a complex model in the two-component regulatory system in Pseudomonas aeruginosa.
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Crystal structures of HptB and the PA1611 receiver domain; Asp565 is the REC phosphoacceptor and phosphotransfer from the PA1611 cytoplasmic part to HptB was shown earlier with radiolabelled ATP.
"between the cytosolic part of PA1611 (a.a. 202–652) and HptB has been confirmed with"
The Diguanylate Cyclase HsbD Intersects with the HptB Regulatory Cascade to Control Pseudomonas aeruginosa Biofilm and Motility.
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PA1611 is one of three hybrid kinases proposed to feed HptB.
"Several upstream sensors have been proposed for the HptB pathway, including PA1611"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods