Gene Ontology annotation through InterPro
Annotation inferred from PAINT phylogenetic analysis
Gene Ontology annotation based on UniProt keywords
Gene Ontology annotation through UniProt subcellular localization
Gene Ontology annotation by Ensembl compara
Reactome pathway annotation
The protein network of HIV budding.
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CHMP2A is part of the class E protein network required for HIV-1 budding.
"dominant-negative mutants of late-acting human class E proteins arrested HIV-1 budding through plasmal and endosomal membranes"
Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1.
The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor.
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hVps24 depletion impairs EGFR degradation and MVB-lysosome fusion.
"Depletion of hVps24 by siRNA showed that this ESCRT subunit, like Tsg101, is important for degradation of the epidermal growth factor (EGF) receptor (EGFR)"
A systematic analysis of human CHMP protein interactions: additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex.
ESCRT-III recognition by VPS4 ATPases.
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VPS4 MIT domain recognizes CHMP2A MIM element.
"the microtubule interacting and transport (MIT) domains of human VPS4A and VPS4B bind conserved sequence motifs located at the carboxy termini of the CHMP1-3 class of ESCRT-III proteins"
Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease.
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ESCRT depletion impairs autophagic degradation and causes ubiquitin-positive aggregate accumulation.
"autophagic degradation is inhibited in cells depleted of ESCRT subunits and in cells expressing CHMP2B mutants, leading to accumulation of protein aggregates containing ubiquitinated proteins"
Helical structures of ESCRT-III are disassembled by VPS4.
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CHMP2A and CHMP3 copolymerize into helical tubular structures.
"We found that the ESCRT-III proteins CHMP2A and CHMP3 (charged multivesicular body proteins 2A and 3) could assemble in vitro into helical tubular structures"
Membrane scission by the ESCRT-III complex.
Defining the membrane proteome of NK cells.
Membrane budding and scission by the ESCRT machinery: it's all in the neck.
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Review of ESCRT-III in MVB formation, viral budding, and cytokinesis.
"ESCRT-III-mediated membrane neck cleavage is crucial for many processes, including the biogenesis of multivesicular bodies, viral budding, cytokinesis, and probably autophagy"
Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance.
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All 11 ESCRT-III proteins required for abscission; CHMP2A depletion causes monopolar spindles.
"We found that depletion of VPS4A, VPS4B, or any of the 11 different human ESCRT-III (CHMP) proteins inhibited abscission"
Syndecan-syntenin-ALIX regulates the biogenesis of exosomes.
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ESCRT-III involved in exosome secretion.
"Syntenin exosomes depend on the availability of heparan sulphate, syndecans, ALIX and ESCRTs"
ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.
ESCRT-III CHMP2A and CHMP3 form variable helical polymers in vitro and act synergistically during HIV-1 budding.
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CHMP2A required for viral release.
"CHMP3 contributes synergistically to HIV-1 budding, and the CHMP3 contribution is ~ 10-fold more pronounced in concert with CHMP2A than with CHMP2B"
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
ESCRT requirements for EIAV budding.
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CHMP2A-VPS4 interaction required for EIAV budding.
"EIAV budding was inhibited by point mutations that abrogate the direct interactions between ALIX:CHMP4B, CHMP4B:CHMP2A, and CHMP2A:VPS4A/B"
ESCRT machinery is required for plasma membrane repair.
Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
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ESCRT-III transiently recruited to reforming NE during late anaphase.
"We propose that ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly"
ESCRT-III controls nuclear envelope reformation.
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CHMP2A is essential for NE sealing.
"The ESCRT-III component charged multivesicular body protein 2A (CHMP2A) is directed to the forming NE through binding to CHMP4B, and provides an activity essential for NE reformation."
LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells.
Structural basis of CHMP2A-CHMP3 ESCRT-III polymer assembly and membrane cleavage.
Deep research review of CHMP2A function
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CHMP2A is a core ESCRT-III subunit required for phagophore closure, cytokinetic abscission, NE sealing, and viral budding.
Reactome pathway - ESCRT-mediated membrane scission
Reactome pathway - ESCRT-III recruitment to midbody
Reactome pathway - Autophagic flux
Reactome pathway - Nuclear envelope sealing
Reactome pathway - ESCRT complex assembly
Reactome pathway - Membrane scission
Reactome pathway - MVB formation
Reactome pathway - Viral budding
Reactome pathway - Plasma membrane repair
Large-scale proteomics and phosphoproteomics of urinary exosomes.
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Structural basis for ESCRT-III protein autoinhibition.
A proteome-scale map of the human interactome network.
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Architecture of the human interactome defines protein communities and disease networks.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly.
Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport.
Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins.
ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate transmembrane cargos in hESC-derived human neurons.
Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15.
Essential role of hIST1 in cytokinesis.