TRIT1 review notes

Deep research

Automated deep research could not be generated: just deep-research-falcon failed
(Edison API 402 Payment Required; perplexity-lite fallback unavailable) and
just deep-research-openai failed with 401. The review uses cached literature and the
UniProt record directly.

Literature used

• Golovko et al. 2000 PMID:11111046 (abstract only).
• Lamichhane et al. 2013 PMID:24126054 - the cytosolic substrate set; mt-tRNA(Ser)UGA and mt-tRNA(Trp) also carry i6A37.
• Yarham et al. 2014, COXPD35 PMID:24901367; dual cytosol/matrix localization by sub-fractionation.
• Schöller et al. 2021 (METTL8) PMID:34774131.

Curation decisions

• ATP binding (InterPro, P-loop) removed: the P-loop of IPTases binds the DMAPP
  pyrophosphate (UniProt BINDING 32..37, ligand dimethylallyl diphosphate); no ATP step.
• nucleic acid binding modified to tRNA binding.
• zinc ion binding kept as non-core (predicted matrin-type zinc finger).
• Two core functions: the same dimethylallyltransferase activity in the mitochondrial
  matrix (mt-tRNAs) and in the cytoplasm (cytosolic tRNA(Ser)/tRNA(Sec)).

Disease context (dismech)

dismech Combined_Oxidative_Phosphorylation_Deficiency_35 used only as a literature lead.