SRP19 PN Consistency Notes

• Generated: 2026-06-18
• Project: PROTEOSTASIS
• Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
• UniProt: P09132
• AIGR review status: COMPLETE
• Review batch: proteostasis-batch-2026-06-11
• Batch change status: added

Source Files Checked

• AIGR review YAML: present - genes/human/SRP19/SRP19-ai-review.yaml
• AIGR review HTML: missing - genes/human/SRP19/SRP19-ai-review.html
• Gene notes: present - genes/human/SRP19/SRP19-notes.md
• GOA TSV: present - genes/human/SRP19/SRP19-goa.tsv
• UniProt record: present - genes/human/SRP19/SRP19-uniprot.txt
• PN dossier: projects/PROTEOSTASIS/reports/phase1_dossiers/SRP19.md
• PN consistency section: projects/PROTEOSTASIS/reports/phase1_dossiers/_sections/SRP19.md
• PN projection report: projects/PROTEOSTASIS/reports/pn_projection/pn_projected_gene_go_summary.tsv
• PN mapping audit: projects/PROTEOSTASIS/reports/pn_mapping_audit/current_mapping_scrutiny.tsv

Deep Research Files

• genes/human/SRP19/SRP19-deep-research-falcon.md

AIGR Review Snapshot

• Description: SRP19 is the 19 kDa protein subunit of the signal recognition particle (SRP), the cytosolic ribonucleoprotein that mediates co-translational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP consists of a single 7SL RNA (~300 nucleotides) and six proteins (SRP9, SRP14, SRP19, SRP54, SRP68, SRP72). SRP19 binds directly to the SRP 7SL RNA and is the key assembly factor of the S domain: it is intrinsically disordered when free and folds upon RNA binding, clamping the apical tetraloops of helices 6 and 8 and thereby remodeling the asymmetric internal loop of helix 8 to create the binding site for the signal-sequence-recognition GTPase SRP54. SRP54 can only bind the SRP RNA after SRP19 has bound (ordered, SRP54-late assembly), so SRP19 is required for incorporation of SRP54 and for productive SRP assembly. SRP19 is an RNA-binding/scaffolding protein, not a GTPase. The mature particle functions in the cytoplasm, but SRP partially assembles in the nucleus/nucleolus, and SRP19 is actively imported into the nucleus by importin 8 and transportin.
• Existing/core annotation action counts: ACCEPT: 21; KEEP_AS_NON_CORE: 6

PN Consistency Summary

• Consistency: Strong and mutually consistent. Deep research, review YAML, and PN annotation all describe SRP19 as the 19 kDa SRP subunit that binds 7SL RNA (clamping helices 6/8), gating SRP54 incorporation and S-domain assembly. The PN "SRP component" label and GO:0006614 mapping match the review's core BP (GO:0006614 IEA, ACCEPT; plus the signal-sequence-recognition child GO:0006617). No contradictions.
• PN story / NEW pressure: PN asserts only the canonical SRP cotranslational-targeting role, already captured (GO:0006614, GO:0006617, GO:0008312 7S RNA binding, GO:0005786 SRP membership). Nuclear/nucleolar assembly localizations (GO:0005730/0005654, kept non-core) and the disease/autoantigen context (PMID:35754847) are in the review but outside the PN story. Conclusion: already captured (no NEW pressure).
• Evidence alignment: PN dossier lists no reference titles; alignment via projected-term provenance. Review's core support (PMID:27899666 SRP72/19 RNA-remodeling structures; PMID:12086622 SRP19 helix-6/8 clamp; PMID:10618370 ordered SRP54-late assembly; PMID:34208095 SRP review) all encode the SRP cotranslational-targeting biology the PN maps to. No divergence.
• Verdict: Fully consistent; PN already captured, review more granular (adds 7S RNA binding and signal-sequence-recognition child). No edits warranted.

Full Consistency Review

• UniProt: P09132 · batch: proteostasis-batch-2026-06-11 · review status: COMPLETE
• PN placement: ER proteostasis|Protein transport|Signal recognition particle component ; PN-node mapping: group=mapped scope=ok_for_propagation_to_go→GO:0006614 (SRP-dependent cotranslational protein targeting to membrane); class Protein transport=mapped→GO:0015031; branch=no_mapping.
• Consistency: Strong and mutually consistent. Deep research, review YAML, and PN annotation all describe SRP19 as the 19 kDa SRP subunit that binds 7SL RNA (clamping helices 6/8), gating SRP54 incorporation and S-domain assembly. The PN "SRP component" label and GO:0006614 mapping match the review's core BP (GO:0006614 IEA, ACCEPT; plus the signal-sequence-recognition child GO:0006617). No contradictions.
• PN story / NEW pressure: PN asserts only the canonical SRP cotranslational-targeting role, already captured (GO:0006614, GO:0006617, GO:0008312 7S RNA binding, GO:0005786 SRP membership). Nuclear/nucleolar assembly localizations (GO:0005730/0005654, kept non-core) and the disease/autoantigen context (PMID:35754847) are in the review but outside the PN story. Conclusion: already captured (no NEW pressure).
• Mapping strategy: No change needed. GO:0006614 (goa_status already_in_goa_exact) is present and ACCEPTed — exact projection, not broader than the review. The class-level GO:0015031 is a broad class target, not asserted of SRP19 specifically.
• Evidence alignment: PN dossier lists no reference titles; alignment via projected-term provenance. Review's core support (PMID:27899666 SRP72/19 RNA-remodeling structures; PMID:12086622 SRP19 helix-6/8 clamp; PMID:10618370 ordered SRP54-late assembly; PMID:34208095 SRP review) all encode the SRP cotranslational-targeting biology the PN maps to. No divergence.
• Verdict: Fully consistent; PN already captured, review more granular (adds 7S RNA binding and signal-sequence-recognition child). No edits warranted.

PN Dossier Context

• review_batch: proteostasis-batch-2026-06-11
• review_yaml: genes/human/SRP19/SRP19-ai-review.yaml
• PN workbook rows: 1

PN row 1: ER proteostasis | Protein transport | Signal recognition particle component

• UniProt: P09132
• In branches: ER
• PN-node mapping records (path + ancestors):
  • [group] ER proteostasis|Protein transport|Signal recognition particle component
    status=mapped scope=ok_for_propagation_to_go GO=[GO:0006614 SRP-dependent cotranslational protein targeting to membrane]
    rationale: This PN group captures core signal-recognition-particle machinery used to direct translating ribosome-nascent chain complexes to the ER membrane. The group is machinery-centric rather than process-equivalent, so it propagates to the GO targeting process.
  • [class] ER proteostasis|Protein transport
    status=mapped scope=ok_for_propagation_to_go GO=[GO:0015031 protein transport]
    rationale: The PN ER Protein transport class groups ER-targeting and ER-insertion pathways. GO protein transport is the appropriate propagation target, while the source class remains ER-specific and broader than any single GO transport subtype.
  • [branch] ER proteostasis
    status=no_mapping scope= GO=[]
    rationale: Reviewed as a top-level PN branch. This is a systems/taxonomy umbrella, not a direct GO assertion; narrower child curations carry any propagating GO mappings.

Projected GO annotations (2)

• GO:0015031 protein transport | scope=ok_for_propagation_to_go | goa_status=new_to_goa | from=ER proteostasis|Protein transport
• GO:0006614 SRP-dependent cotranslational protein targeting to membrane | scope=ok_for_propagation_to_go | goa_status=already_in_goa_exact | from=ER proteostasis|Protein transport|Signal recognition particle component

Note

This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.