Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
The act operon controls the level and time of C-signal production for Myxococcus xanthus development.
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The act operon, four co-transcribed genes at the time of this paper, regulates production of the CsgA C-signal, the morphogen controlling fruiting-body assembly and myxospore differentiation.
"Production of this signal, which is encoded by the csgA gene, is regulated by the act operon of four genes that are co-transcribed from the same start site"
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actC and actD control the timing rather than the magnitude of CsgA production; the actC null mutant raises CsgA 15 h early at the same maximum level.
"The act C and act D genes regulate the time pattern of CsgA production, while achieving the same maximum level"
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The sporulation and aggregation phenotypes reported in the abstract belong to actA and actB, not to actC.
"The act A and act B mutants have the same developmental phenotype: both aggregate, neither sporulates, both prolong rippling"
act operon control of developmental gene expression in Myxococcus xanthus.
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Loss-of-function mutations in each act gene leave C-signal-independent early development untouched, including the time of first expression and the rate of increase of genes expressed up to 3 h.
"Neither the time of first expression nor the rate of expression increase was changed in actA, actB, actC, or actD mutant strains"
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The actC null mutation causes premature expression of aggregation- and sporulation-related reporters, which still reach wild-type levels; actD delays them.
"The other act null mutations caused either premature expression to wild-type levels (actC) or delayed expression (actD), which ultimately rose to wild-type levels"
Mutations of the act promoter in Myxococcus xanthus.
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The actC deletion phenotype reported in PMID:11359579 is premature formation of many small fruiting bodies, resembling a 10-fold overexpression of CsgA.
"deletion of the actC gene led to the premature formation of many small fruiting bodies"
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The authors conclude that ActC inhibits the rise of csgA expression, and propose that the ActD protease degrades ActC to release the inhibition on schedule.
"Since deletion of actC leads to precocious C-signal-dependent gene expression, actC protein appears to inhibit the rise of csgA expression"
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The act operon has five genes, actA to actE, co-expressed as one transcription unit; actE was not part of the four-gene operon described in 2001.
"actE , are expressed together and constitute an operon, because an in-frame deletion of actB decreased expression of actA and actE to the same extent"
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The AF350253 sequence submitted with the 2001 paper was verified against the DK1622 genome sequence CP000113, establishing that the species-level and DK1622-level records describe one organism.
"The entire sequence was verified in the complete sequence of the M. xanthus genome, GenBank accession number CP000113"
UniProt record for actC (Q93P57)
Bioinformatics: UniProtKB entry duplication across two taxon ids for the act operon, and the span of the PTN005180820 graft