Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

PMID:18754794

Identification and characterization of a Jem1p ortholog of Candida albicans: dissection of Jem1p functions in karyogamy and protein quality control in Saccharomyces cerevisiae.

CaJem1p is functional in S. cerevisiae and rescues ER quality control defects.

"CaJem1p localized in the ER when expressed in S. cerevisiae, and expression of CaJem1p from a single-copy plasmid suppressed the temperature sensitive growth and the ER quality control defect of the jem1Deltascj1Delta mutant, indicating that CaJem1p is functional in S. cerevisiae."
CaJem1p can partially suppress karyogamy defects when overexpressed.

"CaJem1p suppressed the karyogamy defect of the jem1Deltamutant only when it was over-expressed from a multicopy plasmid."
The weaker karyogamy function is due to differences in the N-terminal domain that mediates Nep98p interaction.

"Domain-swapping experiments showed that this was due to the difference between the N-terminal domains of ScJem1p and CaJem1p"

file:CANAL/JEM1/JEM1-deep-research-falcon.md

Deep research report on JEM1 (Falcon/Edison Scientific Literature)

JEM1 is the C. albicans Hsp40/J-domain ER co-chaperone (analog of S. cerevisiae Jem1p) that stimulates ER Kar2/BiP ATPase activity to assist ER protein folding and mating-type-specific karyogamy-related events.