Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
The S. pombe translation initiation factor eIF4G is Sumoylated and associates with the SUMO protease Ulp2.
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Purified Ulp2 deconjugates SUMO from high-Mr species (NEM-sensitive) but processes precursor SUMO poorly compared with Ulp1.
"Using assays we described previously [13], we demonstrate that Ulp2 is significantly less able than Ulp1 to process SUMO to the mature form (Figure 1A, lane 2 (Ulp1) and lane 3 (Ulp2)), but is capable of deconjugating SUMO from high Mr species in an N-ethylmaleimide- (NEM)-dependent manner (Figure 1B)."
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Ulp2 is located in predominantly nuclear foci with a minor cytoplasmic fraction, distinct from nuclear-pore-associated Ulp1.
"Thus the location of Ulp2 is distinct from that of Ulp1 [13], indicating that it is unlikely to be part of nuclear pore complexes."
Functional Crosstalk between the PP2A and SUMO Pathways Revealed by Analysis of STUbL Suppressor, razor 1-1.
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Toxicity in ulp2 deletion cells arises from STUbL action on SUMO-chain-modified proteins.
"Therefore, unscheduled STUbL activity on SUMO-chain modified proteins, rather than the SUMO chains themselves, is toxic to ulp2∆ cells."
UniProt entry O13769 (ulp2)