PA1341 (Q9I403) notes
Review session 2026-10-28 (PseudoCAP IBA-seed protocol)
Fetched with just fetch-gene PSEAE PA1341 (no gene symbol; locus tag used as
folder name). Accession Q9I403 matches the worklist (rank 91). Unreviewed
TrEMBL entry (PE 3). No deep-research provider was used. Reviewed alongside
PA1340 (same operon) and for consistency with genes/PSEAE/braF (same
Biolog/growth screen paper) and the Dpp permeases PA4503/PA4504.
Identity
- HisMQ-subfamily ABC permease; PANTHER PTHR30614:SF42 "GLUTAMATE_ASPARTATE
IMPORT PERMEASE PROTEIN GLTJ"; TIGR01726 HEQRo_perm_3TM; four predicted TM
helices; TCDB 3.A.1.3.22.
- Operon PA1342-PA1339 (binding protein, two permeases, ATPase), orthologous to
P. putida KT2440 AatJQMP (PP1071-PP1068) and E. coli GltIJKL. PA1341 is the
GltJ-type permease (P. putida AatQ by operon order); PA1340 is GltK-type.
Literature
- Johnson et al. 2008 (PMID:18833300; full text cached), Biolog PM screen of
transposon mutants (PA1339-PA1341 mutants are in the PAK background):
- PMID:18833300
- Table 2 scores PA1341 only for L-glutamic acid as C source, "C+/-"
(partial). PA1340 additionally shows L-Gln, D-Glu and N-acetyl-L-Glu
phenotypes; the authors note the other two mutants had sub-threshold
reductions on those, "suggesting that this transport system is probably
specific for all four of these substrates".
- Singh and Rohm 2008 (PMID:18310026; abstract only), P. putida ortholog:
- PMID:18310026
- PMID:18310026
- PMID:18310026
Assessment: the PA1341 phenotype is a partial growth defect (indirect), but it is
consistent across all three operon mutants, matches substrate induction, and the
orthologous P. putida system directly takes up Glu. As the permease, PA1341
does part of the transport work. L-glutamate import is sound.
Clade checks (cached PAINT IBD/leaf GAF; QuickGO 2026-10-28)
- PTN004745596 (GO:0051938 L-glutamate import; sole seed PA1341): 7 leaves in 5
taxa: E. coli GltJ (P0AER3), Salmonella GltJ, Yersinia GltJ, Bradyrhizobium
bll7599, blr3097, blr0037, and PA1341. The only experimental annotation among
the other leaves is plasma membrane (IDA) for E. coli GltJ. A GltJ clade of
glutamate/aspartate importer permeases suits the term.
- PTN000770157 (GO:0006865 amino acid transport; 5 seeds: E. coli P0AFT2,
P0AEQ6 and PAO1 Q9I404, Q9I487, Q9I403): 84 leaf IBAs in 28 taxa. A general
amino acid transport term is appropriate for the HisMQ-subfamily permease
clade. (This IBA row is not in the current GOA file for PA1341, presumably
replaced by the more specific L-glutamate import IBA.)
PseudoCAP IBA-seed assessment
| PTN node |
node term |
n_seeds |
leaf IBAs |
seed annotation (term, evidence, PMID) |
verdict |
rationale |
| PTN000770157 |
GO:0006865 amino acid transport |
5 |
84 (28 taxa) |
GO:0051938, IMP, PMID:18833300 (PseudoCAP) |
SOUND |
General term for a HisMQ-subfamily polar amino acid permease clade; other seeds include experimentally characterised E. coli permeases. |
| PTN004745596 |
GO:0051938 L-glutamate import |
1 (sole) |
7 (5 taxa) |
GO:0051938, IMP, PMID:18833300 (PseudoCAP) |
SOUND |
Partial growth defect on L-Glu as C source in a Biolog/plate screen, on its own an indirect phenotype, but consistent across the operon, matched by L-Glu induction, and corroborated by direct Glu/Asp uptake by the orthologous P. putida AatJMQP. The node is a GltJ clade (E. coli/Salmonella/Yersinia GltJ = glutamate/aspartate importers). |
Defects filed: none.
Decisions summary
- ACCEPT: plasma membrane (IBA, IEA).
- MODIFY: L-glutamate import (IBA, IMP), amino acid transmembrane transport
(IEA), transmembrane transport (IEA) -> L-glutamate import across plasma
membrane; amino acid transmembrane transporter activity (IBA) and
transmembrane transporter activity (IEA) -> ATPase-coupled L-glutamate
transmembrane transporter activity; membrane -> plasma membrane; ABC
transporter complex -> substrate-binding subunit-containing ABC transporter
complex.