Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Hint2, a mitochondrial apoptotic sensitizer down-regulated in hepatocellular carcinoma.
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HINT2 is localized to mitochondria
"Hint2 was localized in mitochondria."
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Exhibits adenosine 5'-monophosphoramidase activity
"Hint2 hydrolyzed adenosine monophosphate linked to an amino group (AMP-pNA; k(cat):0.0223 s(-1); Km:128 micromol/L)"
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Catalysis depends on conserved histidine triad
"Hints, histidine triad nucleotide-binding proteins, are adenosine monophosphate-lysine hydrolases"
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Functions as a mitochondrial apoptotic sensitizer
"Exposed to apoptotic stress, fewer HepG2 cells overexpressing Hint2 remained viable"
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Highly expressed in liver and pancreas
"Hint2 was predominantly expressed in liver and pancreas"
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Downregulated in hepatocellular carcinoma
"HINT2 messenger RNA is downregulated in hepatocellular carcinomas"
Hint2 is expressed in the mitochondria of H295R cells and is involved in steroidogenesis.
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HINT2 is expressed in mitochondria of adrenocortical H295R cells
"Hint2 is expressed in the mitochondria of H295R cells"
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HINT2 is involved in steroidogenesis
"this protein is involved in steroidogenesis"
Histidine triad nucleotide-binding proteins HINT1 and HINT2 share similar substrate specificities and little affinity for the signaling dinucleotide Ap4A.
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HINT1 and HINT2 have similar substrate specificity profiles
"Here, a similar substrate specificity profile (kcat /Km ) for model phosphoramidate substrates was found for hHINT2 but with higher kcat and Km values when compared with hHINT1"
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HINT2 has higher kcat and Km values compared to HINT1
"with higher kcat and Km values when compared with hHINT1"
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HINT2 has a broader pH range for maximum catalytic activity
"A broader pH range for maximum catalytic activity was determined for hHINT2 (pK1 = 6.76 ± 0.16, pK2 = 8.41 ± 0.07)"
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Neither HINT1 nor HINT2 bind Ap4A
"Ap4 A was found to have no detectable binding to HINT1 nor HINT2 by isothermal titration calorimetry"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
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HINT2 identified in high-confidence human mitochondrial proteome by quantitative proteomics
"mitochondrial high-confidence proteome of >1,100 proteins (MitoCoP)"
CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system.
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HINT2 detected as CFTR interactor in high-throughput screening (physiological relevance unclear)
"high-throughput screening variant of the Mammalian Membrane Two-Hybrid (MaMTH-HTS) to map the protein-protein interactions"
RHOD binds effectors at the mitochondrial outer membrane
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GTP-bound RHOD binds HINT2 at mitochondrial outer membrane
"GTP-bound RHOD also binds HINT2 at the mitochondrial outer membrane"
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This interaction triggers mitochondrial Ca2+ influx
"which triggers mitochondrial Ca2+ influx"
Deep research summary for HINT2 functional annotation
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HINT2 regulates mitochondrial NAD+ homeostasis and SIRT3-dependent deacetylation
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HINT2 overexpression is cardioprotective after myocardial infarction
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HINT2 is downregulated in hepatocellular carcinoma and colorectal cancer