Use of the ND evidence code for Gene Ontology (GO) terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
RGD ISO annotations to rat from other mammalian species
Molecular recognition of the Tes LIM2-3 domains by the actin-related protein Arp7A.
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Arp7A, TES and Mena form a complex in rat testis extracts; actin, zyxin and profilin I are absent from it.
"immunoblot analysis of Tes immunoprecipitations (IP) with the indicated antibodies reveals that Arp7A, Tes, and Mena are complexed to each other in rat testes extracts"
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The N-terminal extension binds the TES LIM2-3 domains via two apolar-pocket alanines and a GPAK linker.
"Two alanine residues from Arp7A that occupy equivalent apolar pockets in both LIM domains as well as an intervening GPAK linker that binds the LIM2-3 junction are critical for the Arp7A-Tes interaction."
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Arp7A localizes to the subacrosomal layer (acroplaxome) and the Golgi apparatus of round spermatids (mouse testis sections).
"We have now extended these earlier studies and shown that Arp7A is actually localized in the subacrosomal layer, which is also known as the acroplaxome"
Testis-specific actin-like 7A (ACTL7A) is an indispensable protein for subacrosomal-associated F-actin formation, acrosomal anchoring, and male fertility.
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Mouse Actl7a knock-out abolishes subacrosomal F-actin and disrupts acrosome anchoring.
"Significantly, we found a complete loss of subacrosomal filamentous actin (F-actin) structures in knock-out spermatids suggesting a regulatory role for subacrosomal F-actin."
Novel actin-like proteins T-ACTIN 1 and T-ACTIN 2 are differentially expressed in the cytoplasm and nucleus of mouse haploid germ cells.
Disruption in ACTL7A causes acrosomal ultrastructural defects in human and mouse sperm as a novel male factor inducing early embryonic arrest.
Novel bi-allelic variants in ACTL7A are associated with male infertility and total fertilization failure.
Actl7a deficiency in mice leads to male infertility and fertilization failure.
Loss of ACTL7A causes small head sperm by defective acrosome-acroplaxome-manchette complex.
Perinuclear theca protein FNDC8 interacts with CCIN and ACTL7A to ensure proper sperm head shaping during spermiogenesis.
UniProtKB/Swiss-Prot entry Q641W9 (ACL7A_RAT), Actin-like protein 7A, Rattus norvegicus
Manual literature synthesis for rat Actl7a (reviewer-written, no deep-research provider)