Reviewed all 22 existing rows and the core functions with the review and annotation-reviewer workflows. Preserved every machine-sourced term, evidence code, qualifier, source identifier, WITH/FROM entry and row order. No new annotations were added in this audit.
The native S. pombe OTC is mitochondrial, unlike budding-yeast ARG3. PMID:1313366 and native mitochondrial evidence support location. PMID:18849471 links Trx2 to OTC stability/activity without requiring Trx2 catalytic cysteines; this is not automatically a redox catalytic edge. Ornithine binding is retained as a conserved catalytic property, distinguished from a new target-specific binding assay.
Live GO:0000050 describes ornithine-to-arginine synthesis followed by arginine cleavage to urea and regenerated ornithine; it does not require ureotelic nitrogen excretion. PMID:20435771 directly measures S. pombe Car1/Car3 arginase-dependent urea production, and PMID:18849471 explicitly places OTC in the urea-cycle segment. Existing OTC/ASS/ASL rows are retained as non-core participation in this reaction topology, without asserting net full-cycle flux or universal simultaneous operation. The upstream acetylornithine-producing Arg1 is treated separately.
Normal publication caching completed. Normal Falcon research with Perplexity-lite fallback was attempted for this gene; authentication/quota failures prevented a generated report. Manual review used the actual cached primary sources and curated UniProt, and no provider-named research file was fabricated. Per-gene attempts and validation logs are in .cache/module-audit-logs/arginine-SCHPO-arg3-*. Abstract-only or bibliographic-only sources are distinguished from full text in reference reviews; unavailable source details are not treated as experimental refutations.
Actions: ACCEPT 14, KEEP_AS_NON_CORE 1, MODIFY 7. Record sections were backfilled; focused schema/reference validation passed on the initial complete panel. Final targeted checks and HTML rendering are recorded in the audit logs.
Restored supported GO:0005739 mitochondrion rows to ACCEPT under the explicit root-organelle policy; existing matrix rows remain separate. Reasons distinguish organelle screens, direct targeting, publication-linked curated evidence and unavailable full text. No original annotation payload changed. Cytosol decisions use target-specific fractionation/processing evidence rather than relationship qualifiers or generic precursor transit. For Aga1, matrix inference does not imply universal post-import autoproteolysis.
The accepted IBA mitochondrion row retained TERM_SCOPING_PROBLEM / GRANULARITY_MISMATCH metadata from its superseded matrix-refinement decision. Corrected it to NO_FAILURE_CORE, retaining PTN000150427 as the proximate PAINT source with SUPPORTS_TRANSFER. PMID:1313366 distinguishes the native fission-yeast enzyme from predominantly cytoplasmic budding-yeast OTC expressed in S. pombe; independent full-text PMID:32896087 explicitly reports Arg3-GFP mitochondrial localization (Figure EV4). This supports the target's inherited organelle claim without reconstructing the ancestral placement or asserting that every OTC homolog is mitochondrial. Matrix annotations remain separate. No annotation payload, review action, other row or core function changed; four unrelated cytoplasm-to-matrix granularity assessments elsewhere in the panel remain valid and untouched.