Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
RanBPM, Muskelin, p48EMLP, p44CTLH, and the armadillo-repeat proteins ARMC8alpha and ARMC8beta are components of the CTLH complex.
-
ARMC8 alpha and beta are CTLH complex components.
"Here, we used tandem MS with an antibody against RanBPM to purify this complex from a soluble extract of HEK293 cells: we identified Muskelin, p48EMLP, p44CTLH, and the novel armadillo-repeat proteins ARMC8alpha and ARMC8beta as components."
A proteome-scale map of the human interactome network.
Structural and Functional Insights into GID/CTLH E3 Ligase Complexes.
-
ARMC8 is a CTLH scaffold subunit, structurally similar to Gid5.
"In humans, the CTLH complex is named RanBP9 (also known as RanBPM; homologue of Gid1), GID8 (also known as TWA1; homologue of Gid8), and ARMC8 (similar to Gid5) act as the scaffold;"
Exocytosis of tertiary granule lumen proteins
Exocytosis of specific granule lumen proteins
CTLH E3 ligase ubiquitinates LDHA
CTLH E3 ligase ubiquitinates PKM-1
The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation.
-
ARMC8 AP-MS defines the human GID/CTLH complex, which ubiquitinates HBP1.
"Hpb1 protein turnover is regulated by the GID E3 ligase and biochemical evidence suggests that Hbp1 is directly ubiquitinated by the complex, which might contribute to the intermittent proliferation phenotype observed for GID-KO cell lines."
The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation.
-
Human CTLH, including ARMC8 alpha/beta, is an E3 ligase targeting muskelin.
"We confirm that the complex immunoprecipitated from human cells comprises RanBPM, ARMC8 α/β, muskelin, WDR26, GID4 and the RING domain proteins RMND5A and MAEA."
Armc8 is an evolutionarily conserved armadillo protein involved in cell-cell adhesion complexes through multiple molecular interactions.
-
Armc8 is not the strict ortholog of yeast Gid5/Vid28.
"Consequently, Armc8 is not the human ortholog of yeast Gid5/Vid28."
Proteasome-dependent degradation of alpha-catenin is regulated by interaction with ARMc8alpha.
Armadillo Repeat Containing 8alpha Binds to HRS and Promotes HRS Interaction with Ubiquitinated Proteins.
-
ARMc8alpha co-localizes with HRS.
"As demonstrated through immunofluorescence microscopy, ARMc8alpha co-localized with HRS."
Reactome R-HSA-9861563: CTLH E3 ligase ubiquitinates LDHA
Reactome R-HSA-9861640: CTLH E3 ligase ubiquitinates PKM-1
Reactome R-HSA-6798745: Exocytosis of tertiary granule lumen proteins
Reactome R-HSA-6798749: Exocytosis of specific granule lumen proteins
UniProt entry Q8IUR7 (ARMC8_HUMAN)
Affinage mechanistic annotation for ARMC8 (human)