Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Mutations in Fis1 disrupt orderly disposal of defective mitochondria.
-
C. elegans has two Mff homologues (mff-1, mff-2); the Mff double mutant has a mitochondrial fission defect approaching that of drp-1, whereas single mutants are weak (indicating functional redundancy between mff-1 and mff-2)
"mff-1 and mff-2 single mutants have weak effects, and that the Mff double mutant has a mitochondrial fission defect similar to but not as strong as the drp-1 defect"
-
Worm Mff homologues affect both mitochondrial and peroxisome fission, unlike the Fis1 homologues
"We conclude that C. elegans Mff homologues affect mitochondrial and peroxisome fission, whereas Fis1 homologues have no obvious effects."
-
In worm, Mff is not essential for DRP-1 recruitment to mitochondria (a divergence from the mammalian paradigm)
"We conclude that Mff affects fission, but Mff and Fis1 are not essential for fission or for Drp1 recruitment to mitochondria in C. elegans."
-
Loss of Mff reduces the number of mitophagosomes; Mff acts upstream of the Fis1-dependent step in the fission-mitophagy sequence
"significantly reduced number of spots in Mff double and Fis1 Mff quadruple mutant strains"
A novel mitochondrial outer membrane protein, MOMA-1, that affects cristae morphology in Caenorhabditis elegans.
-
mff-1 corresponds to ORF F11C1.2; C. elegans has two Mff homologues encoded by mff-1(F11C1.2) and mff-2(F55F8.6)
"two Mff homologues, encoded by mff-1(F11C1.2) and mff-2(F55F8.6)"
-
MFF-1 is a mitochondrial outer membrane protein, used as an outer membrane marker
"antibodies for EAT-3 (an inner membrane marker), MOMA-1, MFF-1 (an outer membrane marker), and F1β subunit of the ATP synthase complex"
-
In protease-protection of intact mitochondria, MFF-1 is digested without detergent, i.e. it is exposed to the cytosol / anchored in the outer membrane
"MOMA-1 is digested when no detergent is added, like MFF-1, while EAT-3 and F1β are protease protected"
Deep research report for C. elegans mff-1 (falcon/Edison)
-
By orthology to the well-characterized mammalian MFF, MFF-1 is a membrane-anchored receptor/adaptor that recruits DRP-1 to the mitochondrial outer membrane to promote fission; direct functional tests in worm are limited
"MFF-1 functions as a membrane-anchored receptor/adaptor protein that recruits the dynamin-related GTPase DRP-1 from the cytosol to the mitochondrial outer membrane, thereby promoting mitochondrial fission"