Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
UniProtKB record A0A0H2MMT5 (A0A0H2MMT5_VARPD) - Phosphate transport system permease protein PstC from Variovorax paradoxus TBEA6
-
VPARA_10470 (pstC, A0A0H2MMT5) is a 359-aa six-pass inner-membrane ABC permease subunit (InterPro IPR011864, NCBIfam TIGR02138, PANTHER PTHR30425:SF1, PROSITE PS50928) encoded on contig JZWI01000005 (KLN58035.1).
"CC -!- FUNCTION: Part of the binding protein-dependent transport system for
CC phosphate; probably responsible for the translocation of the substrate
CC across the membrane. {ECO:0000256|RuleBase:RU363054}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|RuleBase:RU363054}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU363054}."
Specific amino acid residues in both the PstB and PstC proteins are required for phosphate transport by the Escherichia coli Pst system.
-
Site-directed mutations of conserved Arg237 and Glu240 residues in E. coli PstC (corresponding to Arg276 and Glu280 in VPARA_10470) abolish phosphate transport through the Pst system.
"The amino acid substitutions resulting from the pstC gene mutations, Arg-237----Gln, Glu-240----Gln, or a combination of both, caused the loss of phosphate transport through the Pst system"
Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships.
-
Establishes the sequence and organization of pstC within the phoS-pstC-pstA-pstB-phoU operon.
"The complete sequence is transcribed counterclockwise, in the order phoS pstC pstA pstB phoU."
The PhoU protein from Escherichia coli interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane.
-
PstC and PstA form the integral inner-membrane pore of the PstSCAB ABC phosphate importer.
"PstS is the periplasmic P i -binding protein, PstC and PstA are integral membrane proteins that form the pore through which P i passes, and PstB is the dimeric, cytoplasmic ATPase."